In this work, high-speed micellar electrokinetic chromatography with LIF detection was applied to study the antagonism between three intestinal bacteria, Escherichia coli (E. coli), Bacillus licheniformis (B. licheniformis) and Bacillus subtilis (B. subtilis). The fluorescent derivatization for the bacteria was performed by labeling the bacteria with FITC. In a high-speed capillary electrophoresis (HSCE) device, the three bacteria could be completely separated within 4 min under the separation mode MEKC. The BGE was 1 × TBE containing 30 mM SDS and 1.5 × 10^–5 g/mL polyethylene oxide. The limits of detection for E. coli, B. licheniformis and B. subtilis were 2.80 × 10⁶ CFU/mL, 1.60 × 10⁶ CFU/mL and 1.90 × 10⁶ CFU/mL respectively. Lastly, the method was applied to investigate the antagonism between the three bacteria. The bacteria were mixed and cultured for 7 days. The samples were separated and determined every day to study the interaction between bacteria. The results showed that B. licheniformis and B. subtilis could not inhibit each other, but they could effectively inhibit the reproduction of E. coli. The method developed in this work was quick, sensitive and convenient, and it had great potential in the application of antagonism study for bacteria.

中文翻译：

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高速胶束电动色谱拮抗部分肠道细菌的研究

本工作采用带LIF检测的高速胶束电动色谱法研究了大肠杆菌（E. coli）、地衣芽孢杆菌（B. licheniformis）和枯草芽孢杆菌（B. subtilis）三种肠道细菌的拮抗作用。通过用 FITC 标记细菌来进行细菌的荧光衍生化。在高速毛细管电泳（HSCE）装置中，在MEKC分离模式下，三种细菌可在4分钟内完全分离。BGE 是 1 × TBE，含有 30 mM SDS 和 1.5 × 10 ^–5  g/mL 聚环氧乙烷。大肠杆菌的检测限，地衣芽孢杆菌和枯草芽孢杆菌分别为 2.80 × 10 ⁶ CFU/mL、1.60 × 10 ⁶ CFU/mL 和 1.90 × 10 ⁶ CFU/mL。最后，应用该方法研究了三种细菌之间的拮抗作用。将细菌混合并培养7天。每天对样品进行分离测定，以研究细菌之间的相互作用。结果表明，地衣芽孢杆菌和枯草芽孢杆菌不能相互抑制，但能有效抑制大肠杆菌的繁殖。本工作所开发的方法快速、灵敏、方便，在细菌拮抗研究中具有很大的应用潜力。