Knowledge on soil aggregation and microbial-driven soil C dynamics at the aggregate scale is beneficial for long-term sequestration of C in greenhouse vegetable production (GVP) systems. Here, we used an eight-year fertilization experiment to compare the effects of organic vs. chemical fertilization on soil aggregate stability, as well as living microbial biomass, microbial necromass, and soil C dynamics at the aggregate scale. Relative to chemical fertilization treatment, organic amendments (e.g., manure and/or straw) could improve soil physical quality (as indicated by the value of mean weight diameter), increase microbial biomass and residues, as well as enhance the contributions of microbes to soil organic C (SOC) accumulation within large macroaggregates, small macroaggregates, microaggregates, and silt/clay fractions. Microbial biomass and residues were unevenly distributed among aggregates under different fertilization patterns, i.e., organic amendments made microbial biomass and fungal residues enriched from in silt/clay fractions to in macroaggregates. The low proportions of microbial residue C in SOC in microaggregates demonstrated that the microhabitat of microaggregates limits microbial necromass contributions to SOC accumulation. The changes of microbial biomass were closely related to extractable organic C (EOC), while the variations of fungal and bacterial residues were intimately associated with its corresponding microbes (i.e., fungal and bacterial PLFAs) and enzymes. Moreover, microbial associated ratios (e.g., fungal/bacterial PLFAs) were largely influenced by aggregates and strongly associated with soil chemical associated ratios (e.g., EOC/EON). Our findings provide useful insights on soil microbial-driven C dynamics at the aggregate scale in GVP systems under different fertilization patterns in China.

总体规模的土壤聚集和微生物驱动的土壤碳动力学方面的知识有助于温室蔬菜生产（GVP）系统中碳的长期隔离。在这里，我们使用了一个为期八年的施肥实验，比较了有机施肥和化学施肥对土壤团聚体稳定性以及总团聚体中微生物的生物量，微生物坏死和土壤碳动态的影响。相对于化学施肥处理，有机改良剂（例如粪肥和/或稻草）可以改善土壤物理质量（如平均体重直径的值所示），增加微生物生物量和残留物，以及增强微生物对土壤的贡献有机碳（SOC）在大的大骨料，小的大骨料，微骨料和淤泥/粘土组分中的积累。微生物生物量和残留物在不同施肥模式下在团聚体中分布不均匀，即有机修饰使微生物生物量和真菌残留物从淤泥/粘土部分富集到大团聚体。微生物聚集体中SOC中微生物残留物C的比例低表明，微生物聚集体的微生境限制了微生物坏死对SOC积累的贡献。微生物生物量的变化与可萃取有机碳（EOC）密切相关，而真菌和细菌残留的变化与其相应的微生物（即真菌和细菌PLFA）和酶密切相关。此外，微生物相关比率（例如，真菌/细菌的PLFA）在很大程度上受到聚集体的影响，并与土壤化学相关比率（例如，EOC / EON）密切相关。