Gross nitrification rate (GNR) can reflect the actual status of nitrification process but is difficult to constrain. To develop a practical method for assessing GNR, Δ¹⁷O-NO₃⁻ tracers were applied to container systems under greenhouse conditions to test their feasibility. Two treatments with double- (¹⁵N^Δ17O₃⁻, negative Δ¹⁷O value) or single-labeled (N^Δ17O₃⁻, positive Δ¹⁷O value) fertilizer nitrate salts were implemented to constrain nitrification fractions and GNRs in soilless media container systems. The nitrification fractions calculated from the Δ¹⁷O-NO₃⁻ tracer method concurred with those from the ¹⁵NO₃⁻ tracer method, with small deviations and a linear regression slope close to 1. This suggested the Δ¹⁷O-NO₃⁻ tracer inherently works in the same way as the well-established ¹⁵NO₃⁻ tracer. GNRs in the soilless media container systems ranged from 250.5(±39.1) to 861.5(±275.6) mg N/(m²‧d), generally higher than in natural ecosystems. The discrepancies in GNRs between the two treatments were generally smaller than discrepancies in GNRs between replicate container systems, indicating the applicability of either negatively-labeled or positively-labeled Δ¹⁷O-NO₃⁻ tracers. Commercial nitrate salts mined from the Atacama and Kumtag Deserts are proposed for use as Δ¹⁷O-NO₃⁻ tracers for GNR estimation due to their abundant availability, low cost and distinct discrimination from nitrified NO₃⁻.

总硝化率（GNR）可以反映硝化过程的实际状态，但很难加以约束。为了开发一种实用的方法用于评估GNR，Δ ¹⁷ -O-NO ₃ ^-示踪物施加到容器系统的温室条件下测试其可行性。两种处理用双（¹⁵ Ñ ^Δ17 ö ₃ ^- ，负Δ ¹⁷ O值）或单标记的（N ^Δ17 ø ₃ ^- ，正Δ ¹⁷ O值）肥料硝酸盐实施约束硝化级分和GNR的无土介质容器系统。从Δ计算出的硝化级分¹⁷O形NO ₃ ^-示踪法同意那些从¹⁵ NO ₃ ^-示踪法，具有较小偏差的和的线性回归斜率接近1，所建议的Δ ¹⁷ -O-NO ₃ ^-示踪剂以相同的方式工作固有作为完善的¹⁵ NO ₃ ^-示踪剂。无土培养基容器系统中的GNR范围为250.5（±39.1）至861.5（±275.6）mg N /（m ²‧d），一般高于自然生态系统。在这两种治疗之间的GNR的差异通常比在复制容器系统之间的差异的GNR越小，表示是带负标记的或带正标记Δ的适用性¹⁷ -O-NO ₃ ^-示踪剂。从阿塔卡马和库姆塔格沙漠开采商业硝酸盐被提出用作Δ ¹⁷ -O-NO ₃ ^-示踪剂GNR估计由于它们的丰富可用性，成本低的和不同的判别从NO硝化₃ ^- 。