Many lichen secondary metabolites contributed to the field of pharmacology as an active ingredient of different drugs for years. In the present study, we aimed to test the anticancer activity of olivetoric acid (OA), which we isolated from Pseudevernia furfuracea (L.) Zopf in human hepatocellular carcinoma cells (HepG2). In addition, we used non-tumoral human liver cells (THLE2) to test the level of side effects of OA in vitro. For this purpose, cytotoxic (apoptotic and necrotic), oxidant, genotoxic activities and expression levels of apoptotic genes caused by different concentrations (12.5–400 mg/L) of OA were tested on both cells. Flow cytometric and cytotoxicity tests (MTT and LDH) revealed that OA (100–400 mg/L) had a higher rate of apoptotic effects on HepG2 cells compared to THLE2. Total oxidative stress and oxidative DNA damage levels caused by all concentrations of OA on HepG2 cells was significantly (p < 0.05) higher compared to negative control. Trials with concentrations of 100–400 mg/L significantly (p < 0.05) increased total antioxidant capacity on THLE2 cells compared to the control group. As a result, based on human hepatocellular carcinoma, it is hoped that OA may contribute to the combined or alternative treatment process.

多年来，许多地衣次生代谢产物作为不同药物的活性成分，在药理学领域做出了贡献。在本研究中，我们旨在测试从假单胞菌假单胞菌中分离得到的橄榄酸（OA）的抗癌活性。（L.）人肝癌细胞（HepG2）中的Zopf。此外，我们使用非肿瘤性人类肝细胞（THLE2）在体外测试OA的副作用水平。为此，在这两种细胞上测试了由不同浓度（12.5-400 mg / L）的OA引起的细胞毒性（细胞凋亡和坏死性），氧化剂，遗传毒性活性和凋亡基因的表达水平。流式细胞仪和细胞毒性试验（MTT和LDH）显示，与THLE2相比，OA（100-400 mg / L）对HepG2细胞具有更高的凋亡率。 与阴性对照组相比，所有浓度的OA对HepG2细胞造成的总氧化应激和氧化DNA损伤水平均显着（p <0.05）。浓度为100–400 mg / L的试验显着（p <0.05）与对照组相比，THLE2细胞的总抗氧化能力增加了。结果，基于人肝细胞癌，希望OA可能有助于联合或替代治疗过程。