Advances in microfluidics for zebrafish processing are critical to facilitate the bioassay in new drug development. Still, a manipulation platform for zebrafish relies mainly on the “static” agarose material and is coupled with the conventional fish facility to ensure the time-history investigation with the homogenous zebrafish population for drug screening. A fully automated IoT (Internet of Things) microfluidics system is necessary to be developed to enable smart and high-speed zebrafish testing without the need for fish facility support. In this work, a smart microfluidic device was presented to enable (i) highly efficient temperature control with the precision of ± 0.1 °C, (ii) remote zebrafish transport through light patterning, and (iii) perfusion and dynamic culturing of zebrafish all together assembled for on-chip high-quality imaging. A new microscale manipulation is envisaged to initiate a new chapter of running bioassays.

斑马鱼加工微流体技术的进步对于促进新药开发中的生物测定至关重要。尽管如此，用于斑马鱼的操纵平台仍主要依靠“静态”琼脂糖材料，并与传统的鱼类设施相结合，以确保对同质斑马鱼种群进行时程调查以进行药物筛选。需要开发一个全自动的IoT（物联网）微流体系统，以实现智能和高速的斑马鱼测试，而无需鱼类设施的支持。在这项工作中，提出了一种智能微流控设备，该设备能够（i）以±0.1°C的精度进行高效的温度控制，（ii）通过光图案进行斑马鱼的远程运输，以及（iii）斑马鱼的灌注和动态培养组装用于片上高质量成像。