Soybean is a major economic crop worldwide, but its yield and quality are greatly affected by root-knot nematode infection. This study aimed to assess the effects of Meloidogyne javanica parasitism on soybean growth, yield, and quality. Soybean plants were inoculated with 0, 1000, 2000, or 4000 eggs + second-stage juveniles (J2) of M. javanica and evaluated for vegetative and nematode parameters. Grains from each treatment were analyzed for yield, proximate composition, total phenolic content, total flavonoid content, isoflavone profile, and antioxidant capacity. Plants free of nematodes had lower vegetative growth and higher yield than nematode-infected plants. The maximum estimated reproduction factor was 34.85, achieved by inoculation of 2433 eggs + J2. Moisture, fiber, and protein contents decreased with increasing inoculum levels. Lipid content increased with inoculum level until reaching 22.59 g 100 g−1 of sample. Total phenolic and flavonoid contents decreased with increasing inoculum level up to about 2000 eggs + J2 and then increased until reaching 219.20 mg gallic acid equivalents 100 g−1 of sample and 121.67 mg quercetin equivalent 100 g−1 of sample, respectively, at 4000 eggs + J2. A similar behavior was observed for antioxidant capacity determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging, superoxide radical scavenging, and ferric reducing antioxidant power assays. The highest contents of malonylgenistin (539 mmol g−1), acetylglycitin (106 mmol g−1), and genistin (87 mmol g−1) were found in grains from plants inoculated with 4000 eggs + J2. M. javanica inoculum level affected soybean development, grain production, yield, composition, and antioxidant capacity.

中文翻译：

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影响爪哇根结线虫寄生大豆的发展和化学成分

大豆是世界范围内的主要经济作物，但其产量和质量受到根结线虫感染的极大影响。这项研究旨在评估棉铃虫对大豆生长，产量和品质的影响。大豆植物分别接种0、1000、2000或4000个鸡蛋+爪哇M. javanica的第二阶段幼虫（J2），并评估其营养和线虫参数。分析每种处理的谷物的收率，附近组成，总酚含量，总黄酮含量，异黄酮特性和抗氧化能力。与线虫感染的植物相比，不含线虫的植物的营养生长较低，产量更高。通过接种2433枚鸡蛋+ J2可获得的最大估计繁殖因子为34.85。水分，纤维和蛋白质含量随着接种量的增加而降低。脂质含量随接种量的增加而增加，直至达到22.59 g 100 g-1的样品。总酚和类黄酮含量随着接种量的增加而降低，直至约2000个蛋+ J2，然后一直增加，直到4000个蛋分别达到219.20 mg没食子酸当量100 g-1样品和121.67 mg槲皮素当量100 g-1样品。 + J2。通过2，2-二苯基-1-吡啶甲基肼基自由基清除，超氧化物自由基清除和三价铁还原抗氧化剂能力测定法确定的抗氧化剂容量具有相似的行为。在接种4000个鸡蛋+ J2的植物的谷物中发现了丙二醛生成素（539 mmol g-1），乙酰甘氨酸（106 mmol g-1）和染料木素（87 mmol g-1）的最高含量。M. javanica接种物水平影响大豆发育，谷物产量，产量，组成，