As a water-soluble carbon monoxide-releasing molecule, CORM-3 is widely used as a CO donor to study CO in the life system. CORM-3 can also replace gaseous CO as a therapeutic drug molecule to reveal the physiological and pathological effects of CO in life. Therefore, it is of great importance to visualize and track CORM-3 in the life system. We develop herein a near-infrared (NIR) fluorescent probe CORM3-NIR that can detect CORM-3 both in living cells and in vivo effectively. The probe is based on the unique fluorescent QCy7 and uses a 4-nitrobenzyl group to trap CORM-3, and importantly, it shows good water solubility and responds rapidly, selectively, and sensitively to CORM-3, releasing QCy-7 and producing distinct colorimetric and significant NIR fluorescence change signals at 743 nm. The Stokes shift is up to 81 nm. The probe is also able to detect CORM-3 ratiometrically with fluorescence at 743 and 600 nm. Besides, with low cytotoxicity, the probe also shows good NIR fluorescence bioimaging ability for CORM-3 in live cells and mice, which indicates that CORM3-NIR is an effective probe for tracking and studying CORM-3 in the life system.

中文翻译：

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近红外成像探针对一氧化碳释放分子-3的体内成像和跟踪

作为一种可释放一氧化碳的水溶性分子，CORM-3被广泛用作研究生命系统中一氧化碳的一氧化碳供体。CORM-3还可以替代气态CO作为治疗性药物分子，以揭示CO在生活中的生理和病理作用。因此，对生命系统中的CORM-3进行可视化和跟踪非常重要。我们在这里开发了一种近红外（NIR）荧光探针CORM3-NIR，它可以在活细胞和体内检测到CORM-3有效。该探针基于独特的荧光QCy7，并使用4-硝基苄基捕获CORM-3，重要的是，它显示出良好的水溶性并对CORM-3迅速，选择性和敏感地响应，从而释放QCy-7并产生独特的比色和显着的NIR荧光在743 nm处发生变化。斯托克斯位移最大为81 nm。该探针还能够按比例检测743和600 nm处的荧光的CORM-3。此外，由于具有低细胞毒性，该探针还对活细胞和小鼠的CORM-3具有良好的近红外荧光生物成像能力，这表明CORM3-NIR是在生命系统中追踪和研究CORM-3的有效探针。