Seed germination events modulate microbial community composition, which ultimately influences seed-to-seedling growth performance. Here, we evaluate the germinated maize (variety SHS 5050) root bacterial community of disinfected seed (DS) and non-disinfected seed (NDS). Using a gnotobiotic system, sodium hypochlorite (1.25%; 30 min)-treated seeds showed a reduction of bacterial population size and an apparent increase of bacterial community diversity associated with a significant selective reduction of Burkholderia-related sequences. The shift in the bacterial community composition in DS negatively affects germination speed, seedling growth and reserve mobilization rates compared with NDS. A synthetic bacterial community (syncom) formed by 12 isolates (9 Burkholderia spp., 2 Bacillus spp., and 1 Staphylococcus sp.) obtained from natural microbiota maize seeds herein was capable of recovering germination and seedling growth when reintroduced in DS. Overall, results showed that changes in bacterial community composition and selective reduction of Burkholderia-related members' dominance interfere with germination events and the initial growth of the maize. By cultivation-dependent and -independent approaches, we deciphered seed–maize microbiome structure, bacterial niches location and bacterial taxa with relevant roles in seedling growth performance. A causal relationship between seed microbial community succession and germination performance opens opportunities in seed technologies to build-up microbial communities to boost plant growth and health.

中文翻译：

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玉米发芽过程中种子传播细菌组的结构和作用的见解

种子萌发事件调节微生物群落组成，最终影响种子到幼苗的生长性能。在这里，我们评估了消毒种子 (DS) 和未消毒种子 (NDS) 的发芽玉米（品种 SHS 5050）根细菌群落。使用生菌系统，次氯酸钠（1.25%；30 分钟）处理的种子显示出细菌种群大小的减少和与伯克霍尔德氏菌相关序列的显着选择性减少相关的细菌群落多样性的明显增加。与 NDS 相比，DS 中细菌群落组成的变化对发芽速度、幼苗生长和储备动员率产生负面影响。由 12 个分离株（9 个伯克霍尔德氏菌属、2 个芽孢杆菌属和 1 个葡萄球菌属）形成的合成细菌群落 (syncom)。) 从本文中的天然微生物群玉米种子中获得的种子在重新引入 DS 时能够恢复发芽和幼苗生长。总体而言，结果表明细菌群落组成的变化和伯克霍尔德氏菌相关成员优势的选择性降低会干扰玉米的发芽事件和初始生长。通过依赖培养和不依赖培养的方法，我们破译了种子-玉米微生物组结构、细菌生态位位置和与幼苗生长性能相关的细菌分类群。种子微生物群落演替与发芽性能之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。总体而言，结果表明细菌群落组成的变化和伯克霍尔德氏菌相关成员优势的选择性降低会干扰玉米的发芽事件和初始生长。通过依赖培养和不依赖培养的方法，我们破译了种子-玉米微生物组结构、细菌生态位位置和与幼苗生长性能相关的细菌分类群。种子微生物群落演替与发芽性能之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。总体而言，结果表明细菌群落组成的变化和伯克霍尔德氏菌相关成员优势的选择性降低会干扰玉米的发芽事件和初始生长。通过依赖培养和不依赖培养的方法，我们破译了种子-玉米微生物组结构、细菌生态位位置和与幼苗生长性能相关的细菌分类群。种子微生物群落演替与发芽性能之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。通过依赖培养和不依赖培养的方法，我们破译了种子-玉米微生物组结构、细菌生态位位置和与幼苗生长性能相关的细菌分类群。种子微生物群落演替与发芽性能之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。通过依赖培养和不依赖培养的方法，我们破译了种子-玉米微生物组结构、细菌生态位位置和与幼苗生长性能相关的细菌分类群。种子微生物群落演替与发芽性能之间的因果关系为种子技术提供了建立微生物群落以促进植物生长和健康的机会。