Abstract

Cleaning and disinfection protocols are not always able to remove biofilm microbes present in breweries, indicating that novel anti-biofilm strategies are needed. The preventive activities of three in-house synthesized members of the 2-aminoimidazole class of anti-biofilm molecules were studied against 17 natural brewery biofilms and benchmarked against 18 known inhibitors. Two 2-aminoimidazoles belonged to the top six inhibitors, which were retested against 12 defined brewery biofilm models. For the three best inhibitors, tannic acid (n° 1), 2-aminoimidazole imi-AAC-5 (n° 2), and baicalein (n° 3), the effect on the microbial metabolic activity was evaluated. Here, the top three inhibitors showed similar effectiveness, with baicalein possessing a slightly higher efficacy. Even though the 2-aminoimidazole was the second-best inhibitor, it showed a lower biocidal activity than tannic acid, making it less prone to resistance evolution. Overall, this study supports the potential of 2-aminoimidazoles as a preventive anti-biofilm strategy.

摘要

清洁和消毒方案并不总是能够去除啤酒厂中存在的生物膜微生物，这表明需要新颖的抗生物膜策略。研究了2-氨基咪唑类抗生物膜分子的三个内部合成成员对17种天然啤酒生物膜的预防活性，并针对18种已知抑制剂进行了基准测试。两个2-氨基咪唑属于前六种抑制剂，它们已针对12种确定的啤酒生物膜模型进行了重新测试。对于三种最佳抑制剂，单宁酸（n°1），2-氨基咪唑imi-AAC-5（n°2）和黄ical素（n°3），评估了对微生物代谢活性的影响。在此，排名前三位的抑制剂显示出相似的功效，其中黄ical素的功效略高。尽管2-氨基咪唑是第二好的抑制剂，它显示出比单宁酸更低的杀菌活性，使其不易产生抗药性。总的来说，这项研究支持了2-氨基咪唑作为一种潜在的抗生物膜策略的潜力。