Outbreaks of lesser spruce sawfly, Pristiphora abietina (Christ) (Hymenoptera: Tenthredinidae), on conifers regularly occur in Turkey and other European countries. Chemical pesticides have been used to contain outbreaks, but those may damage the forest and disturb natural enemies. To minimize the use of chemical pesticides in the management of this forest pest, we identified the entomopathogenic fungi (EPF) that naturally occur on P. abietina and tested their efficacy against the pest under laboratory and semi‐field conditions. Using morphological and molecular techniques, 13 EPF isolates were obtained from the pest and identified as Beauveria bassiana (Bals.‐Criv.) Vuill., Beauveria pseudobassiana S.A. Rehner & Humber, and Lecanicillium muscarium (Petch.) Zare & W. Gams. A laboratory screening test at 10⁶ conidia ml⁻¹ showed that all isolates caused 59‐100% mortality after 14 days. Further experiments were performed with the three most effective isolates, all belonging to B. bassiana. For these isolates, an increase in conidia dose increased pest mortality. Also, the ability of horizontal transmission in the pest population was shown. All three isolates were effective in killing P. abietina on spruce under semi‐field conditions. These data will contribute to the development of an integrated pest management program for P. abietina based on microbial biological control.

中文翻译：

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与云杉较小的锯蝇Pristiphora abietina相关的昆虫病原真菌的生物多样性和致病性

土耳其和其他欧洲国家/地区经常在针叶树上爆发较小云杉的锯齿蝇（长翅目：天蛾科）。化学杀虫剂已被用来控制疫情的爆发，但是这些杀虫剂可能会破坏森林并扰乱天敌。为了最大程度地减少化学农药在森林病虫害的管理中的使用，我们鉴定了在假单胞菌自然存在的昆虫病原真菌（EPF），并在实验室和半田间条件下测试了它们对害虫的功效。使用形态学和分子技术，从该害虫中获得了13种EPF分离株，并被鉴定为球孢白僵菌（Balveria bassiana（Bals.Criv。）Vuill。），球孢白僵菌SA Rehner＆Humber和Lecanicillium muscarium（Petch。）Zare＆W. Gams。在10 ⁶分生孢子ml ^-1下进行的实验室筛选测试表明，所有分离株在14天后均导致59-100％的死亡率。对三个最有效的分离株进行了进一步的实验，所有分离株均属于球孢杆菌。对于这些分离株，分生孢子剂量的增加会增加害虫的死亡率。此外，还显示了有害生物种群中水平传播的能力。在半田间条件下，所有三种分离株均能有效杀死云杉中的假单胞菌。这些数据将有助于为一体的综合虫害管理程序的开发P.藓基于微生物的生物控制。