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Nanohybrid composed of graphene oxide functionalized with sodium hyaluronate accelerates bone healing in the tibia of rats
Biomaterials Advances ( IF 7.9 ) Pub Date : 2021-02-12 , DOI: 10.1016/j.msec.2021.111961
Paulo César de Lacerda Dantas , Paulo Antônio Martins-Júnior , Danielle Carvalho Oliveira Coutinho , Vanessa Barbosa Andrade , Thalita Marcolan Valverde , Erick de Souza Ávila , Tatiane Cristine Silva Almeida , Celso Martins Queiroz-Junior , Marcos Augusto Sá , Alfredo Miranda Góes , Luiz Orlando Ladeira , Anderson José Ferreira , Leandro Silva Marques

This study synthesized and characterized a nanohybrid composed of graphene oxide (GO) functionalized with sodium hyaluronate (HY) (GO-HY), evaluated its effect in vitro and determined its osteogenic potential in vivo. The synthesized nanohybrid was analyzed by Scanning electron microscopy (SEM), Raman spectrometry, Thermogravimetry, Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction. MC3T3-E1 cell viability was assessed by MTT assay in 48 and 72 h. Bone defects were created in tibia of 40 Wistar rats and filled with blood clot (control), 1% HY, GO (50, 100 and 200 μg/mL) and the nanohybrid (50, 100 and 200 μg/mL). After 7 and 14 days, histomorphometric analysis was carried out to assess osteogenic potential of the nanohybrid. Immunohistochemical analysis evaluated the expression of vascular endothelial growth factor (VEGF) in bone defects. Thermogravimetric analysis, Raman and FTIR spectrometry confirmed the functionalization of GO with HY by covalent bonds. Five μg/mL concentrations of the nanohybrid did not alter the viability of the MC3T3-E1 cells. Histomorphometric analysis demonstrated that the nanohybrid at 100 μg/mL significantly accelerated the bone repair in tibia of rats when compared to controls (p < 0.01). Immunohistochemical analysis showed a significantly less intense VEGF expression in tibia treated with the nanohybrid when compared to controls (p < 0.05). The nanohybrid composed of GO functionalized with HY was able to induce the acceleration of the tissue regeneration process in bone defects created in the tibia of rats. This novel nanohybrid is a promising material for the field of bone tissue engineering.



中文翻译:

由透明质酸钠官能化的氧化石墨烯组成的纳米复合物可加速大鼠胫骨的骨愈合

这项研究合成并表征了由透明质酸钠(HY)(GO-HY)功能化的氧化石墨烯(GO)组成的纳米杂交在体外评估了其效果并确定了其在体内的成骨潜力。通过扫描电子显微镜(SEM),拉曼光谱,热重分析,傅立叶变换红外光谱(FTIR)和X射线衍射分析合成的纳米杂化物。通过MTT分析在48和72小时中评估MC3T3-E1细胞生存力。在40只Wistar大鼠的胫骨中形成了骨缺损,并充满了血凝块(对照),1%HY,GO(50、100和200μg/ mL)和纳米杂交体(50、100和200μg/ mL)。7和14天后,进行组织形态计量学分析以评估纳米杂交体的成骨潜力。免疫组织化学分析评估了骨缺损中血管内皮生长因子(VEGF)的表达。热重分析,拉曼光谱和FTIR光谱证实了共价键可将GO与HY官能化。浓度为5μg/ mL的纳米杂交体不会改变MC3T3-E1细胞的生存能力。组织形态计量学分析表明,与对照组相比,100μg/ mL的纳米杂交体显着促进了大鼠胫骨的骨修复(p  <0.01)。免疫组织化学分析显示,与对照组相比,经纳米杂化处理的胫骨中VEGF的表达明显降低(p  <0.05)。由HY功能化的GO组成的纳米杂交体能够诱导大鼠胫骨中产生的骨缺损组织再生过程的加速。这种新型的纳米杂化材料是骨组织工程领域中很有希望的材料。

更新日期:2021-02-23
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