Local anesthetics (LAs) are capable of influencing cell viability in systemic immunity and may also modify metabolism of those present in umbilical cord blood (UCB) following obstetric neuraxial analgesia and anaesthesia. Data regarding UCB immature cells, important for the neonate and critical for putative UCB transplantations, are lacking. LAs are capable of stimulating intracellular nitric oxide (NO) in human neutrophils; no information is available concerning newly perpetuated cells and its potential association with viability. The study aimed at assessing the LAs influence on the cell viability and intracellular NO production by UCB CD34+CD133– and CD34+ CD133+ cell populations. Mononuclear cells separated from UCB samples (n = 19) were incubated with bupivacaine (0.0005, 0.005, 1 mM), lidocaine (0.002, 0.02, 4 mM), and ropivacaine (0.0007, 0.007, 1.4 mM) for 4 h. Flow cytometry was applied for the assessment of cell viability and intracellular NO generation in CD34+CD133– and CD34+CD133+ cell populations using annexinV/7-AAD and DAF-2DA stainings, respectively. CD34+CD133+ cells showed less pronounced late apoptosis and necrosis as compared to CD34+CD133-population. Intracellular NO generation was comparable between both cell populations studied. LAs neither influenced cell viability nor changed NO production in either population. LAs do not interfere with viability and intracellular NO generation in the UCB CD34+CD133– and CD34+CD133+ cell populations.

中文翻译：

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暴露于局部麻醉药的脐带血CD34 + CD133–和CD34 + CD133 +细胞群体的活力和细胞内一氧化氮生成

局部麻醉剂（LAs）能够影响全身免疫中的细胞活力，并且还可能会改变产科神经痛和麻醉后脐带血（UCB）中存在的那些物质的代谢。缺乏关于UCB未成熟细胞的数据，该数据对于新生儿很重要，并且对于假定的UCB移植至关重要。洛杉矶能够刺激人类嗜中性粒细胞的细胞内一氧化氮（NO）。没有信息有关新永存的细胞及其与生存能力的潜在联系。该研究旨在评估UCB CD34 + CD133–和CD34 + CD133 +细胞群体对LAs对细胞活力和细胞内NO产生的影响。从UCB样品（n = 19）中分离出的单核细胞与布比卡因（0.0005，0.005，1 mM），利多卡因（0.002，0.02，4 mM）和罗哌卡因（0.0007，0）孵育。007，1.4mM）4小时。流式细胞仪分别使用AnnexinV / 7-AAD和DAF-2DA染色法评估CD34 + CD133–和CD34 + CD133 +细胞群体的细胞活力和细胞内NO生成。与CD34 + CD133种群相比，CD34 + CD133 +细胞显示的晚期凋亡和坏死不太明显。在研究的两个细胞群之间，细胞内NO的产生是可比较的。洛杉矶既不影响细胞活力也不改变任何一个群体中的一氧化氮产生。LAs不会干扰UCB CD34 + CD133-和CD34 + CD133 +细胞群的活力和细胞内NO的产生。与CD34 + CD133种群相比，CD34 + CD133 +细胞显示的晚期凋亡和坏死不太明显。在研究的两个细胞群之间，细胞内NO的产生是可比较的。洛杉矶既不影响细胞活力也不改变任何一个群体中的一氧化氮产生。LAs不会干扰UCB CD34 + CD133-和CD34 + CD133 +细胞群的活力和细胞内NO的产生。与CD34 + CD133种群相比，CD34 + CD133 +细胞显示的晚期凋亡和坏死不太明显。在研究的两个细胞群之间，细胞内NO的产生是可比较的。洛杉矶既不影响细胞活力也不改变任何一个群体中的一氧化氮产生。LAs不会干扰UCB CD34 + CD133-和CD34 + CD133 +细胞群的活力和细胞内NO的产生。