Abstract

This experiment was conducted to assess the effect of Chaya (Cnidoscolus aconitifolius) leaf pellet (CHYP) on rumen fermentation, nutrients digestibility and microbial protein synthesis in growing crossbred bulls. Four animals, with an average liveweight of 160 ± 10 kg were randomly arranged in a 4 × 4 Latin square design. There were four treatments: 0, 4, 6 and 8% CHYP supplementation of DMI. The study findings showed that increasing supplementation level of CHYP linearly enhanced (p < 0.05) DM, OM, and CP digestibilities. Rumen characteristics, namely NH₃–N concentration and bacterial population were increased (p < 0.05) while, protozoal and fungal population remained unchanged, as level of CHYP supplementation increased. Total rumen volatile fatty acids (VFA) and propionic acid (C₃) were enhanced (p < 0.05). Furthermore, N utilisation especially N absorption, N retention and efficiency of microbial nitrogen synthesis (EMNS) were significantly improved by increasing level of CHYP supplementation. Hence, CHYP supplementation is highly promising for ruminant feeding.

• HIGHLIGHTS

• Chaya leaf pellet (CHYP) contains high level of crude protein, essential amino acids and minerals.

• CHYP supplementation increased nutrient digestibility and propionic acid (C₃) in rumen.

• CHYP enhanced N–balance, microbial nitrogen supply (MNS) and efficiency of microbial nitrogen synthesis (EMNS).

摘要

进行该实验以评估Chaya（Cnidoscolus aconitifolius）叶小丸（CHYP）对成长中的杂种公牛瘤胃发酵，养分消化率和微生物蛋白质合成的影响。以4×4拉丁方形设计将四只平均活重为160±10公斤的动物随机排列。有四种治疗方法：DMI的0、4、6和8％CHYP补充。研究结果表明，增加CHYP的添加水平线性地提高了（p  <0.05）DM，OM和CP的消化率。瘤胃特性，即NH ₃ -N浓度和细菌数量增加（p <0.05），而随着CHYP补充水平的增加，原生动物和真菌种群保持不变。总瘤胃挥发性脂肪酸（VFA）和丙酸（C ₃）增强（p <  0.05）。此外，通过增加CHYP的添加量，氮的利用率，特别是氮的吸收，氮的保留和微生物氮合成的效率得到了显着提高。因此，CHYP补充剂对于反刍动物的饲喂非常有前途。

• 强调

• 查亚叶小丸（CHYP）包含高含量的粗蛋白，必需氨基酸和矿物质。

• CHYP补充提高了瘤胃中的营养物质消化率和丙酸（C ₃）。

• CHYP增强了氮平衡，微生物氮供应（MNS）和微生物氮合成效率（EMNS）。