Biodegradable compounds can cause undesired microbial growth in drinking water systems and these compounds can originate from the water or pipe materials used in drinking water systems. The aim of our study was to determine the influence of different pipe materials on the microbial populations in water and biofilm under semi-stagnant conditions. The microbial communities in biofilm and water, which were in contact with seven different materials, were characterized by determining ATP concentrations, microbial composition gene copy numbers of some specific microbial groups. The ATP concentration in water and biofilm varied between the different materials with glass (negative control) < copper < PVCC < PE-Xc < PE-Xb < PE-100 < PVC-P. Gene copy numbers of Legionella spp., Mycobacterium spp., Pseudomonas spp., Aeromonas spp., fungi and Vermamoeba vermiformis were also higher for PVC-P and PE than for glass, copper and PVCC. The bacterial community composition in water and biofilm varied between materials as well. PERMANOVA and CAP analysis demonstrated that copper and PVC-P are different when compared to the other materials. Furthermore, bacterial community composition and ATP concentrations in water and biofilm were similar after eight and 16 weeks incubation, but differed from results obtained after one week. Finally, the ATP, the specific microbial groups and the bacterial community composition also differed between water and biofilm on each material. We conclude from our study that pipe material is an important factor that influences the biomass concentration, abundance of specific microorganisms and the bacterial community composition in distribution systems with unchlorinated drinking water.

可生物降解的化合物会导致饮用水系统中微生物的生长，这些化合物可能源自饮用水系统中使用的水或管道材料。我们研究的目的是确定半停滞条件下不同管道材料对水和生物膜中微生物种群的影响。通过确定某些特定微生物群的ATP浓度，微生物组成基因拷贝数来表征与7种不同物质接触的生物膜和水中的微生物群落。在不同材料中，玻璃和水之间的ATP浓度变化（阴性对照）<铜<PVC C <PE-Xc <PE-Xb <PE-100 <PVC-P。军团菌属的基因拷贝数，PVC-P和PE的分枝杆菌属，假单胞菌属，气单胞菌属，真菌和Vermamoeba vermiformis的含量也比玻璃，铜和PVC高C.水和生物膜中的细菌群落组成也因材料而异。PERMANOVA和CAP分析表明，与其他材料相比，铜和PVC-P是不同的。此外，孵育八周和十六周后，细菌群落组成和水和生物膜中的ATP浓度相似，但与一周后获得的结果不同。最后，每种材料上的水和生物膜之间的ATP，特定的微生物基团和细菌群落组成也有所不同。从我们的研究中我们得出结论，管道材料是影响无氯饮用水分配系统中生物量浓度，特定微生物的丰度和细菌群落组成的重要因素。