In this paper, we prepared a low cost, environmentally friendly and stable surface enhanced fluorescence (SEF) substrate, namely, Ag@razor clam (Ag@RC), for fluorescence enhancement via magnetron sputtering technique. When the magnetron sputtering time was 10 min (Ag-10), we obtained optimal fluorescence enhancement effect for this substrate. The maximum enhancement factor (EF) for rhodamine 6G (R6G) was 1574 with small relative standard deviation (RSD). Above results indicate that this SEF substrate possesses strong EF, excellent stability and repeatability. In order to better understand the experimental results, the three-dimensional (3D) finite-difference time-domain (FDTD) method is used to simulate the electric field distribution of Ag-10 substrate and further study the enhancement effect of fluorescence. Finally, we find that Ag-10 substrate is applied not only to enhance fluorescence intensity of R6G molecules, but also to improve that of a variety of fluorescence molecules, which provides a new inspiration on the multiple molecules detection.

在本文中，我们准备了一种低成本，环保且稳定的表面增强荧光（SEF）基板，即Ag @ razor clam（Ag @ RC），用于通过磁控溅射技术增强荧光。当磁控管溅射时间为10分钟（Ag-10）时，我们获得了该基板的最佳荧光增强效果。罗丹明6G（R6G）的最大增强因子（EF）为1574，相对标准偏差（RSD）小。以上结果表明该SEF基材具有很强的EF，出色的稳定性和可重复性。为了更好地理解实验结果，我们使用三维时域有限差分法（FDTD）来模拟Ag-10衬底的电场分布，并进一步研究荧光的增强效果。最后，