Objective

To investigate the protective effect of phosphatidylserine liposomes (PSL) on post-stroke (ST) injuries such as neuroinflammation and depression in mice.

Methods

Brain ischemia was induced via the right unilateral common carotid artery occlusion model. Then, behavioral assessments including the forced swimming test (FST) and tail suspension test (TST) were used to evaluate the antidepressant-like effect of PSL. Moreover, inflammatory cytokines changes in the hippocampus including TNF-α and IL-10 levels as well as the number of survived neurons were evaluated in ST mice using immunohistochemistry (IHC).

Results

A significant reduction of the immobility time in both behavioral tests indicated the antidepressant activity of PSL. Moreover, the number of viable neurons increased significantly with PSL treatment, which was similar to control group, compared to the untreated ST group. IHC analysis of ST mice receiving PSL showed a significant reduction in TNF-α and IL-10 levels in the inflamed hippocampus of mice.

Conclusion

Oral PSL may improve post-stroke depression (PSD) through its anti-inflammatory properties.

中文翻译：

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神经保护性磷脂酰丝氨酸脂质体通过减轻小鼠海马中的神经炎症减轻与中风有关的抑郁样行为

客观的

调查磷脂酰丝氨酸脂质体（PSL）对中风（ST）损伤，如小鼠神经炎症和抑郁症的保护作用。

方法

通过右侧单侧颈总动脉闭塞模型诱发脑缺血。然后，使用行为评估包括强迫游泳测试（FST）和尾部悬吊测试（TST）来评估PSL的抗抑郁样作用。此外，使用免疫组织化学（IHC）在ST小鼠中评估了海马中炎性细胞因子的变化，包括TNF-α和IL-10水平，以及存活神经元的数量。

结果

在两种行为测试中，固定时间的显着减少表明PSL具有抗抑郁活性。此外，与未治疗的ST组相比，PSL治疗后的存活神经元数量显着增加，与对照组相似。对接受PSL的ST小鼠进行的IHC分析表明，发炎的海马体中TNF-α和IL-10水平明显降低。

结论

口服PSL可通过其抗炎特性改善中风后抑郁症（PSD）。