Cooking with active oxygen and solid alkali (CAOSA) is a mild and environmentally friendly method for separation of lignin components. The degradation mechanism of hemicellulose associated with this method has been investigated. It was found that the hemicellulose-derived polysaccharides were directly oxidized rather than hydrolyzed to monosaccharides for degradation. To avoid the undesirable degradation of hemicellulose components by CAOSA, the idea of separating hemicellulose first in the form of xylan to produce xylooligosaccharide was presented by this study. Although hydrothermal autocatalysis has been commonly used in hemicellulose separation, the effects of acid-enhanced hydrothermal method and alkali-suppressed hydrothermal method on hydrothermal autocatalysis were systematically compared for the first time, and it was found that the acid-enhanced method was beneficial for highly selective extraction of xylan. Then, the hemicellulose-removed bamboo was treated with CAOSA process to separate lignin from cellulose. It was found that solid alkali dosage of CAOSA could be evidently decreased for hemicellulose-removed bamboo, which was helpful to reduce the cost of CAOSA. Coupling hemicellulose pre-extraction and CAOSA lignin fractionation strategy, a comprehensive biorefinery case for bamboo biomass could be anticipated.

用活性氧和固体碱（CAOSA）烹饪是分离木质素成分的温和且环保的方法。已经研究了与此方法相关的半纤维素的降解机理。已经发现，半纤维素衍生的多糖被直接氧化而不是水解成单糖以进行降解。为避免CAOSA对半纤维素成分的不良降解，本研究提出了先以木聚糖的形式分离半纤维素以产生木寡糖的想法。尽管半纤维素分离中普遍使用水热自催化，但首次系统地比较了酸增强水热法和碱抑制水热法对水热自催化的影响，并且发现酸增强方法对于木聚糖的高度选择性提取是有益的。然后，用CAOSA工艺处理除去半纤维素的竹子，以从纤维素中分离出木质素。发现去除半纤维素的竹子可以显着降低CAOSA的固体碱用量，这有助于降低CAOSA的成本。结合半纤维素的预提取和CAOSA木质素的分馏策略，可以预期一个全面的生物精制案例，用于竹生物量。