Diseases caused by phytopathogenic microorganisms are difficult to control and can affect plants at different stages of their development. Several resistance genes and antimicrobial peptides (AMPs) have been identified and related to the resistance process of Capsicum. In recent years, studies have shown that peppers, especially the accession UENF1381, present resistance against phytopathogenic microorganisms. This work aimed identify and characterize AMPs of the leaf and root from Capsicum annuum L. UENF1381 and to analyze the inhibitory activity of the AMPs on different enzyme families and valuated the inhibitory activity on Colletotrichum scovillei. Initially, self-fertilized pepper seeds were cultured for 45 days in glass flasks containing ½-MS medium. Then, leaves were inoculated with inoculum of Xanthomonas euvesicatoria (10⁸ CFU mL⁻¹) or water (control). Leaf and root samples were collected at 12, 24 and 48 h after inoculation to extraction. The activity of extracts on different enzyme families was analyzed by incubating the enzymes (trypsin, α-amylase and β-1,3-glucanase) with 30 μg mL⁻¹ of the obtained extracts. The effect of extracts on fungi was also tested. It was identified that protein of leaf and root extracts from C. annuum L. UENF1381 presented a majority of bands with a low molecular mass (6–14 kDa). We observed that all leaf and root extracts significantly inhibited trypsin and α-amylase activity and able to significantly inhibit C. scovillei growth. With this work we hope to contribute to the use of peptides as potential molecules in microbial control and in the development of new Capsicum cultivars resistant to microorganisms.

由植物致病性微生物引起的疾病难以控制，并可能影响植物处于不同发育阶段的疾病。已经鉴定了几种抗性基因和抗菌肽（AMPs），它们与辣椒的抗性过程有关。近年来，研究表明，辣椒，特别是登录号UENF1381，表现出对植物病原微生物的抗性。这项工作旨在鉴定和表征辣椒辣椒叶片UENF1381的根和根，并分析其对不同酶家族的抑制活性，并评估其对炭疽菌的抑制活性。首先，将自受精的胡椒种子在装有½-MS培养基的玻璃烧瓶中培养45天。然后，叶接种黄单胞 菌（10 ⁸  CFU mL ^-1）或水（对照）的接种物。接种后第12、24和48小时收集叶和根样品。通过将酶（胰蛋白酶，α-淀粉酶和β-1,3-葡聚糖酶）与30μgmL ^-1的提取物一起孵育来分析提取物对不同酶家族的活性。还测试了提取物对真菌的作用。鉴定出来自C.annuum的叶和根提取物的蛋白质L. UENF1381呈现了大多数低分子量（6-14 kDa）的谱带。我们观察到，所有叶和根提取物均显着抑制胰蛋白酶和α-淀粉酶的活性，并能够显着抑制斯科氏梭菌的生长。通过这项工作，我们希望能够将肽作为潜在分子用于微生物控制和开发新的对微生物具有抗性的辣椒品种。