当前位置: X-MOL 学术Tree Physiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular mechanisms for magnesium-deficiency-induced leaf vein lignification, enlargement and cracking in Citrus sinensis revealed by RNA-Seq
Tree Physiology ( IF 4 ) Pub Date : 2020-10-26 , DOI: 10.1093/treephys/tpaa128
Xin Ye 1 , Hui-Yu Huang 1 , Feng-Lin Wu 1 , Li-Ya Cai 1 , Ning-Wei Lai 1 , Chong-Ling Deng 2 , Jiu-Xin Guo 1 , Lin-Tong Yang 1 , Li-Song Chen 1
Affiliation  

Abstract
Citrus sinensis (L.) Osbeck seedlings were fertigated with nutrient solution containing 2 [magnesium (Mg)-sufficiency] or 0 mM (Mg-deficiency) Mg(NO3)2 for 16 weeks. Thereafter, RNA-Seq was used to investigate Mg-deficiency-responsive genes in the veins of upper and lower leaves in order to understand the molecular mechanisms for Mg-deficiency-induced vein lignification, enlargement and cracking, which appeared only in the lower leaves. In this study, 3065 upregulated and 1220 downregulated, and 1390 upregulated and 375 downregulated genes were identified in Mg-deficiency veins of lower leaves (MDVLL) vs Mg-sufficiency veins of lower leaves (MSVLL) and Mg-deficiency veins of upper leaves (MDVUL) vs Mg-sufficiency veins of upper leaves (MSVUL), respectively. There were 1473 common differentially expressed genes (DEGs) between MDVLL vs MSVLL and MDVUL vs MSVUL, 1463 of which displayed the same expression trend. Magnesium-deficiency-induced lignification, enlargement and cracking in veins of lower leaves might be related to the following factors: (i) numerous transciption factors and genes involved in lignin biosynthesis pathways, regulation of cell cycle and cell wall metabolism were upregulated; and (ii) reactive oxygen species, phytohormone and cell wall integrity signalings were activated. Conjoint analysis of proteome and transcriptome indicated that there were 287 and 56 common elements between DEGs and differentially abundant proteins (DAPs) identified in MDVLL vs MSVLL and MDVUL vs MSVUL, respectively, and that among these common elements, the abundances of 198 and 55 DAPs matched well with the transcript levels of the corresponding DEGs in MDVLL vs MSVLL and MDVUL vs MSVUL, respectively, indicating the existence of concordances between protein and transcript levels.


中文翻译:

RNA-Seq揭示镁缺乏导致柑桔叶片静脉木质化,扩大和开裂的分子机制

摘要
用含有2 [镁(Mg)足够]或0 mM(Mg不足)Mg(NO 32的营养液对柑桔(Osbeck)奥斯贝克幼苗施肥。持续16周。此后,使用RNA-Seq研究上下叶静脉中的缺镁反应基因,以了解缺镁诱导的静脉木质化,扩大和破裂的分子机制,这种机制仅出现在下叶中。在这项研究中,在下叶的Mg缺乏静脉(MDVLL)与下叶的Mg缺乏静脉(MSVLL)和上叶的Mg缺乏静脉中鉴定出3065个上调和1220个下调以及1390个上调和375个下调基因( MDVUL)vs上部叶片的镁充足静脉(MSVUL)。MDVLL与MSVLL和MDVUL与MSVUL之间共有1473个共同的差异表达基因(DEG),其中1463个表现出相同的表达趋势。镁缺乏引起的木质化,下叶脉的增大和破裂可能与以下因素有关:(i)木质素生物合成途径,细胞周期调控和细胞壁代谢调控中涉及的许多转录因子和基因被上调;(ii)激活了活性氧,植物激素和细胞壁完整性信号。蛋白质组和转录组的联合分析表明,在MDVLL vs MSVLL和MDVUL vs MSVUL中分别鉴定出DEG和差异丰富蛋白(DAP)之间共有287和56个共同元素,在这些共同元素中,有198和55个DAP的丰度分别与MDVLL vs MSVLL和MDVUL vs MSVUL中相应DEG的转录水平匹配,表明蛋白质和转录水平之间存在一致性。
更新日期:2020-10-26
down
wechat
bug