Abstract

Malathion (MT) is one of the most widely used organophosphorus insecticides which induces toxicity through oxidative stress induction, free radical production and acetylcholinesterase inhibition. In this work, HepG2 cells were used to determine the effect of Zataria multiflora methanolic extract (MEZM) and rosmarinic acid (RA) on MT-induced cytotoxicity, oxidative stress, and apoptosis. Total phenolic content (TPC) and total flavonoid content (TFC) were determined and plant was further standardized based on RA content using HPLC method. The cultured HepG2 cells were pretreated with MEZM (1 μg/ml) and RA (0.1 μg/ml) for 4 h and exposed to MT (100 μM). Cell viability, oxidative stress biomarkers, ROS production, and cell death were examined after 24 h. The amount of RA was determined 73.48 mg/g dried extract. IC₅₀ values of MEZM and MT were 368.56 μg/ml and 99.43 μM, respectively. Pretreatment with MEZM and RA decreased the cytotoxicity, oxidative stress, and cell percentage in the late apoptosis and necrosis stages induced by MT. There was no significant difference between MEZM and RA effects. The present study showed the significant protective effects of MEZM against toxicity induced by MT in hepatocytes which can be attributed to the plant antioxidant constituents including RA.

摘要

马拉硫磷（MT）是使用最广泛的有机磷杀虫剂之一，可通过氧化应激诱导，自由基产生和乙酰胆碱酯酶抑制作用诱导毒性。在这项工作中，HepG2细胞用于确定何首乌甲醇提取物（MEZM）和迷迭香酸（RA）对MT诱导的细胞毒性，氧化应激和凋亡的影响。测定总酚含量（TPC）和总黄酮含量（TFC），并使用HPLC方法根据RA含量进一步标准化植物。将培养的HepG2细胞用MEZM（1μg/ ml）和RA（0.1μg/ ml）预处理4 h，然后暴露于MT（100μM）。24小时后检查细胞活力，氧化应激生物标志物，ROS产生和细胞死亡。确定RA的量为73.48mg / g干提取物。我知道了MEZM和MT的₅₀值分别为368.56μg/ ml和99.43μM。MEZM和RA预处理可降低MT诱导的晚期凋亡和坏死阶段的细胞毒性，氧化应激和细胞百分比。MEZM和RA效果之间无显着差异。本研究表明，MEZM对MT诱导的肝细胞毒性具有显着的保护作用，这可归因于包括RA在内的植物抗氧化剂成分。