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Possible role of WRKY transcription factors in regulating immunity in Oryza sativa ssp. indica
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2021-02-10 , DOI: 10.1016/j.pmpp.2021.101623
Arsheed H. Sheikh , Rana M. Fraz Hussain , Naheed Tabassum , Raghuram Badmi , Sylvestre Marillonnet , Dierk Scheel , Justin Lee , Alok Sinha

Plants have developed a robust transcription machinery to combat potential pathogenic organisms. One of the hallmarks of early immune responses is the activation of the WRKY transcription factors post infection. Specific WRKYs proteins from Arabidopsis are known substrates of MAPK pathway to mediate the flg22 elicited early immunity.

In the current study, using the Golden Gate cloning strategy, we aim to clone the entire WRKY transcription factor family from Oryza sativa ssp. indica consisting of more than 100 members and study their MAPK interaction and subsequent role in PTI. Using a reporter LUC assay in protoplasts we investigated the early defense responses in a few interesting OsWRKY candidates.

Interestingly, we observed stringent regulation of WRKY expression in cells and their transcriptional expression only under specific stress responses. The phenomenon of gene expression regulation by intron retention (IR) was prevalently observed in rice WRKY transcripts. We could show the role of WRKY8, 24, and 77 in early defense responses. It was observed that WRKY24 enhanced the expression of early defense response marker genes like NHL10 while WRKY8 and WRKY77 supressed their expression.

This study highlights the complicated mechanism by which OsWRKYs expression is possibly regulated and the distinctive roles of some individual members in plant immunity. At the same time this study serves as a cautionary warning for plant researchers to be mindful of the intron retention mechanism while cloning OsWRKYs.



中文翻译:

在调节免疫的WRKY转录因子可能作用水稻亚种。印度

植物已经开发出强大的转录机制来对抗潜在的病原生物。早期免疫反应的标志之一是感染后WRKY转录因子的激活。来自拟南芥的特定WRKYs蛋白是MAPK途径的已知底物,可介导flg22引起的早期免疫。

在当前研究中,我们使用金门克隆策略,旨在从稻(Oryza sativa ssp)中克隆整个WRKY转录因子家族。of由100多个成员组成,研究其MAPK相互作用以及其在PTI中的作用。在原生质体中使用记者LUC分析,我们调查了一些有趣的OsWRKY候选人的早期防御反应。

有趣的是,我们仅在特定的应激反应下观察到细胞中WRKY表达的严格调控及其转录表达。在水稻WRKY转录物中普遍观察到通过内含子保留(IR)调节基因表达的现象。我们可以证明WRKY8、24和77在早期防御反应中的作用。观察到WRKY24增强了诸如NHL10的早期防御反应标记基因的表达,而WRKY8和WRKY77则抑制了它们的表达。

这项研究强调了可能通过调控OsWRKYs表达的复杂机制,以及某些个体成员在植物免疫中的独特作用。同时,这项研究为植物研究人员在克隆OsWRKY时注意内含子保留机制提供了警告

更新日期:2021-02-28
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