Bacterial surface display system has been adopted in various biotechnological applications. In the case of Bacillus subtilis, most of the studies have been developed using spore based surface display system utilizing the inherent rigidity of spore against heat, alkali, and shear stress. But, spore harvest, purification and separation need additional cost and labor. To eliminate this procedure and to use the gram-positive nature of B. subtilis, YuaB, which is one of the major B. subtilis biofilm components and locates in the cell wall, based cell surface display system, is developed. P43 promoter driven overexpression of YuaB-His₆ tag does not hamper bacterial cell growth and promoted biofilm formation of recombinant strain. Flow cytometry of recombinant strain and its protoplast using FITC-Anti His₆ antibody, verified that YuaB locate in plasma membrane and protrude to the outside of cell wall, which means YuaB can be used as very efficient anchoring motif. Using surface expressed YuaB-His₆ tag, removal of divalent metal ion, Cu²⁺ and Ni²⁺, was tried to test its possibility for the environmental application of developed system.

细菌表面展示系统已在各种生物技术应用中采用。在枯草芽孢杆菌的情况下，大多数研究都是使用基于孢子的表面展示系统开发的，该系统利用孢子对热、碱和剪切应力的固有刚性。但是，孢子的收获、纯化和分离需要额外的成本和劳动力。为了消除这一过程并利用枯草芽孢杆菌的革兰氏阳性特性，开发了YuaB，它是主要的枯草芽孢杆菌生物膜成分之一，位于细胞壁中，基于细胞表面显示系统。P43 启动子驱动 YuaB-His _{6的过表达}标签不阻碍细菌细胞生长并促进重组菌株的生物膜形成。_{使用FITC-Anti His 6}抗体对重组菌株及其原生质体进行流式细胞仪检测，证实YuaB位于质膜内并突出到细胞壁外，这意味着YuaB可以作为非常有效的锚定基序。使用表面表达的YuaB-His ₆标签，去除二价金属离子Cu ²⁺和Ni ²⁺，尝试测试其在开发系统的环境应用中的可能性。