The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

本研究旨在制造一种新的壳聚糖 - 胶原蛋白海绵（CCS），用于潜在的伤口敷料应用。CCS 由 3.0% 壳聚糖与 1.0% I 型胶原蛋白 (7:3(w/w)) 的混合物通过冷冻干燥制成。然后准备敷料以通过一系列测试评估其性能。这种新型敷料证明了其对 NIH3T3 细胞的安全性。此外，与由大肠杆菌和金黄色葡萄球菌接种的空对照相比，CCS 显示出显着的环绕抑制区. 此外，CCS 的水分率比胶原蛋白和空白海绵组增加得更快。结果表明，CCS具有无毒、生物相容性好、抗菌活性好、保水性好等特点。我们使用全层切除伤口愈合模型来评估新敷料的体内功效。结果显示，与仅使用胶原蛋白作为阴性对照以及仅使用壳聚糖治疗的损伤相比，术后第 14 天的愈合显着。我们的结果表明，壳聚糖 - 胶原伤口敷料被确定为进一步伤口应用的新的有希望的候选物。