To date, coagulation tests are unable to reflect in vivo coagulation status in the same system, including platelet function, fibrin clot formation, and whole blood flow. The Total Thrombus Analysis System (T-TAS), which is a microfluidic assay that simulates conditions in vivo, measures whole blood flow at defined shear rates under conditions designed to assess platelet function (PL-chip) or coagulation and fibrin clot formation (AR-chip). The T-TAS records occlusion start time, occlusion time, and area under the curve. We evaluated this test in healthy control dogs. We also investigated the effect in vivo of acetylsalicylic acid (ASA), and the effect in vitro of an anticoagulation drug (dalteparin; low-molecular-weight heparin; LMWH). The CV of the AUC of both chips was good (CVs of 6.45% [PL] and 1.57% [AR]). The inhibition of platelet function by ASA was evident in the right-shift in the PL test pressure curve. The right-shift in the AR test pressure curves showed that the administration of LMWH inhibited both platelets and the coagulation cascade. The T-TAS may be useful in the evaluation of canine blood coagulation.

迄今为止，凝血测试无法反映同一系统中的体内凝血状态，包括血小板功能、纤维蛋白凝块形成和全血流量。总血栓分析系统 (T-TAS) 是一种模拟体内条件的微流体分析，在设计用于评估血小板功能 (PL-chip) 或凝血和纤维蛋白凝块形成 (AR) 的条件下以规定的剪切速率测量全血流量-芯片）。T-TAS 记录遮挡开始时间、遮挡时间和曲线下面积。我们在健康的对照犬中评估了这项测试。我们还研究了乙酰水杨酸 (ASA) 的体内作用，以及抗凝药物（达肝素；低分子量肝素；LMWH）的体外作用。两种芯片的 AUC 的 CV 都很好（CV 为 6.45% [PL] 和 1.57% [AR]）。ASA对血小板功能的抑制在PL测试压力曲线的右移中很明显。AR测试压力曲线的右移表明LMWH的施用抑制了血小板和凝血级联。T-TAS 可用于评估犬的血液凝固。