Abstract

Soybean soaking water whey (SWW) is obtained as the waste of soy milk production and mostly represents an environmental problem. The aim of this study was to assess the content of proteins and content and activity of trypsin inhibitors of fresh SWW, obtained during soy milk production. Two zones of Bowman-Birk trypsin inhibitors (BBI) were detected. One was identified as a monomeric form of BBI (0.61-2.93%) and the other one was identified as a polymeric form of BBI (0.45-3.33%). The degree of BBI extraction (1.88-5.49%) was influenced by the soybean genotype and the grain size, i.e. it increased with increasing grain size. Kunitz trypsin inhibitor was not detected. Total proteins were found in traces in SWW (0.03-0.06%). Low residual trypsin inhibitor activity (0.32-0.55%) suggested that SWW can potentially be applied for preparing food or feed. In that case it will not be waste but a cheap functional supplement with BBI as a biologically active component.

摘要

大豆浸泡水乳清（SWW）是作为豆浆生产的浪费而获得的，主要代表了环境问题。这项研究的目的是评估在豆浆生产过程中获得的新鲜SWW的蛋白质含量以及胰蛋白酶抑制剂的含量和活性。检测到Bowman-Birk胰蛋白酶抑制剂（BBI）的两个区域。一种被鉴定为BBI的单体形式（0.61-2.93％），另一种被鉴定为BBI的聚合物形式（0.45-3.33％）。BBI提取率（1.88-5.49％）受大豆基因型和粒大小的影响，即随着粒大小的增加而增加。未检测到Kunitz胰蛋白酶抑制剂。在SWW中的痕量中发现了总蛋白（0.03-0.06％）。胰蛋白酶抑制剂的低残留活性（0.32-0.55％）表明SWW可以潜在地用于制备食物或饲料。