The effectiveness of single versus combined dietary supplementation of multi-strain probiotics and exogenous multi-enzyme (Ronozyme^®) on growth indices, feed utilization and gastrointestinal functions of Siberian sturgeon was investigated for 10 weeks. A feeding trial was performed in triplicate with the juveniles (N = 240; 138.1 g), which were fed on C: basal diet (control), E: 62.5 mg/kg Ronozyme^® (25 mg/kg xylanase, 30 mg/kg protease and 7.5 mg/kg phytase), P: 2 g/kg probiotics mixture (Pediococcus pentosaceus 1 × 10⁹ and Lactococcus lactis 1 × 108 CFU/g) and P + E diets. Upon the completion of the feeding trial, the group fed with dietary P + E showed the highest final body weight and metabolic weight gain; however, the lowest feed conversion ratio was calculated in E and P + E groups (p < 0.05). Protein and lipid efficiency ratios were significantly decreased in the control group (p < 0.05). The highest and lowest crude protein and lipid contents were recorded in P + E treatment, respectively (p < 0.05). The height, width and area of intestinal villi in the fish fed with treated diets were higher than the control group (p < 0.05). The activities of lipase and protease were increased in P and P + E groups (p < 0.05). The load of lactic acid bacteria was upregulated in the fish gut fed with P and P + E diets (p < 0.05). Overall, the combination of the probiotics mixture and multi-enzyme significantly improved the metabolism and digestibility of Siberian sturgeon.

中文翻译：

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多菌株益生菌和多酶的单一或联合饮食管理影响西伯利亚鲟（Acipenser baerii）的生长、身体成分、消化酶活性和肠道形态

10 周研究了单一与联合膳食补充多菌株益生菌和外源多酶 (Ronozyme ^® ) 对西伯利亚鲟生长指数、饲料利用率和胃肠功能的有效性。甲饲养试验一式三份与青少年（执行Ñ 基础日粮（对照），E：62.5毫克/千克RONOZYME，将其供给第C;138.1克= 240）^®（25毫克/千克木聚糖酶，30mg / kg的蛋白酶和 7.5 mg/kg 植酸酶），P：2 g/kg 益生菌混合物（戊糖片球菌1 × 10 ⁹和乳酸乳球菌1 × 108 CFU/g) 和 P + E 饮食。饲喂试验完成后，饲喂 P+E 日粮的组显示出最高的最终体重和代谢增重；然而，最低饲料转化率是在 E 和 P + E 组中计算的 ( p  < 0.05)。对照组的蛋白质和脂质效率比显着降低（p  < 0.05）。P+E处理分别记录了最高和最低的粗蛋白和脂质含量（p  < 0.05）。饲喂治疗组的鱼肠绒毛的高度、宽度和面积均高于对照组（p  < 0.05）。P组和P+E组脂肪酶和蛋白酶活性均升高（p < 0.05)。在饲喂 P 和 P + E 饲料的鱼肠道中，乳酸菌的含量上调 ( p  < 0.05)。总的来说，益生菌混合物和多酶的组合显着提高了西伯利亚鲟鱼的新陈代谢和消化率。