Silkworm (Bombyx mori) pupa protein (SPP) is a high-quality source of animal protein with substantial nutritional benefits and health value. To develop an efficient extraction method for SPP that is environmentally friendly, we selected choline hydroxide ionic liquid (CH-IL) as the extraction solvent and performed orthogonal experiments to optimize the extraction conditions. We demonstrated that 3% CH-IL, a solid-to-liquid ratio (g/mL) of 1:30, an extraction temperature of 40 °C, and an extraction time of 1 h facilitated the most efficient extraction. Compared to the conventional alkali solubilization–acid precipitation method, the CH-IL extraction increased protein content by 12.14%. Protein structure analysis showed that the β-sheet content increased by 10.98% and that of disulfide bonds reduced by 16.4%. The processing properties of the CH-IL extracted protein showed that the solubility, emulsification, and foaming capacity were enhanced by 82.87%, 15.44%, and 18.97%, respectively. The physical properties of SPP remarkably improved relative to the increased stretching of the polypeptide chains. The findings of this study provide technical knowledge that will enhance the processing performance of pupal proteins.

蚕（家蚕蛋白（SPP）是高质量的动物蛋白来源，具有丰富的营养价值和健康价值。为了开发一种对环境友好的高效SPP提取方法，我们选择了氢氧化胆碱离子液体（CH-IL）作为提取溶剂，并进行了正交试验以优化提取条件。我们证明了3％的CH-IL，固液比（g / mL）为1:30，萃取温度为40°C，萃取时间为1 h促进了最有效的萃取。与传统的碱溶-酸沉淀法相比，CH-IL萃取使蛋白质含量提高了12.14％。蛋白质结构分析表明，β-折叠含量增加了10.98％，二硫键减少了16.4％。CH-IL提取蛋白的加工性能表明其溶解度，乳化和起泡能力分别提高了82.87％，15.44％和18.97％。相对于多肽链增加的拉伸，SPP的物理性质显着改善。这项研究的发现提供了将增强that蛋白加工性能的技术知识。