Immortelle (Helichrysum italicum (Roth) G. Don), also known as curry plant, is a herbaceous perennial plant with documented biological properties that contribute to its attractiveness and use in the cosmetic, pharmaceutical, and food industry. Availability of DNA markers can significantly improve the knowledge of complex genetics and biochemistry of this aromatic plant. To improve in-depth genetic studies, high-throughput sequencing of a genomic DNA has been performed and a set of 24 SSR markers was developed. In total, 17,025,076 high quality reads were assembled in contigs with the cumulative length of 258.6 Mbp. Genome-wide microsatellite sequences were identified in 8.19% (36,882) contigs, among which dinucleotide (71.2%) and trinucleotide (24.4%) repeats were the most abundant. Twenty-four finally selected microsatellite loci (including di- and tri-nucleotides) which exhibited polymorphism with unambiguous and reproducible amplification products, were used for detailed genetic diversity analysis on a set of 28 H. italicum samples. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on the Jaccard index differentiated 27 genotypes into two main clusters according to their geographical origin (Northern and Southern Adriatic region), whereas the commercially available plant did not cluster with other H. italicum individuals and morphological evaluation confirmed that it belongs to Helichrysum angustifolium (Lam.) DC. The great diversity of H. italicum samples from the Adriatic observed by new microsatellites will serve for selection of most promising genotypes for propagation and their implementation in agricultural production. The first set of microsatellites developed in the present study provides a valuable resource of DNA markers for further breeding programs, germplasm characterization, and cross-species transferability studies.

蜡菊（Helichrysum italicum（Roth）G. Don），也称为咖喱植物，是一种多年生草本植物，具有已证明的生物学特性，有助于其吸引力，并在化妆品，制药和食品工业中使用。DNA标记物的可用性可以大大提高这种芳香植物的复杂遗传学和生物化学知识。为了改善深入的遗传研究，已对基因组DNA进行了高通量测序，并开发了24种SSR标记。共有17,025,076个高质量读段组装在重叠群中，累积长度为258.6 Mbp。在8.19％（36,882）重叠群中鉴定出全基因组微卫星序列，其中二核苷酸（71.2％）和三核苷酸（24.4％）重复序列最多。H. italicum样品。基于Jaccard指数的算术平均非加权对分组法（UPGMA）根据其地理起源（北亚得里亚海南部和南部亚得里亚海地区）将27个基因型分为两个主要的聚类，而市售植物并未与其他意大利胡麻聚类。个体和形态学评估证实其属于蜡菊属植物（Helichrysum angustifolium（Lam。）DC）。意大利ali的多样性新的微卫星观测到的来自亚得里亚海的样本将用于选择最有希望的基因型进行繁殖并在农业生产中应用。在本研究中开发的第一套微卫星为进一步的育种计划，种质鉴定和跨物种转移性研究提供了宝贵的DNA标记资源。