Lupeol is the main active constituent of Hygrophila schulli (HS) plant. A simple, reliable and reproducible method of quantification is needed for the quality control of HS. Therefore, high-performance thin-layer chromatography (HPTLC) method was established for determining lupeol in HS roots and aerial parts. The laboratory isolated and identified (by UV, FT-IR, 1H- and 13C-NMR and MS) lupeol from HS roots was used to develop the proposed method. The method was validated in terms of linearity, limits of detection and quantification, stability, precision, accuracy and robustness studies. Aluminum-backed HPTLC plates precoated with silica gel 60F₂₅₄ were used as the stationary phase and benzene‒chloroform‒methanol (93:5.75:1.25, V/V) as the optimized mobile phase. Post-chromatographic derivatization was done with anisaldehye‒H₂SO₄ reagent, and the chromatograms were scanned using CAMAG TLC Scanner III at 540 nm. The linear regression analysis data for the calibration plots of lupeol showed good linearity relationship with y = 7.1841x + 751.42 (r² = 0.9979) regression equation in the concentration range of 200–1000 ng band⁻¹ (R_F = 0.43 ± 0.02). Mean ± SD values of slope and intercept were 7.1840 ± 0.067 and 751.42 ± 45.50, respectively. The stability study suggested that lupeol in chloroform was stable within 24 h at room temperature (%RSD = 1.07). The average percentage recovery of lupeol from the petroleum ether extracts of HS roots and aerial parts was 98.66 ± 0.65 and 98.77 ± 0.39, respectively, with a % w/w content 5.02 ± 0.23 and 0.39 ± 0.11% w/w on dry weight basis. The limits of detection and quantification were found to be 17.85 and 54.11, respectively.

羽扇豆酚是Schgro（Hygrophila schulli）植物的主要活性成分。HS的质量控制需要一种简单，可靠且可重现的定量方法。因此，建立了高效薄层色谱法（HPTLC）来测定HS根和地上部分中的羽扇豆酚。从HS根中分离并鉴定（通过UV，FT-IR，1H-和13C-NMR和MS）的紫杉醇用于开发该方法。该方法在线性，检测和定量限，稳定性，精度，准确性和鲁棒性研究方面得到了验证。预涂硅胶60F _254的铝底HPTLC板用作固定相，苯‒氯仿‒甲醇（93：5.75：1.25，V / V）作为优化的流动相。色谱分析后的衍生化是用茴香醛H ₂ SO ₄试剂进行的，色谱图使用CAMAG TLC Scanner III在540 nm扫描。 在200-1000 ng band ^-1浓度范围内（R _F  = 0.43±0.02）， 卢贝醇校准图的线性回归分析数据与y = 7.1841 x  + 751.42（r ² = 0.9979）回归方程显示出良好的线性关系。。斜率和截距的平均值±SD值分别为7.1840±0.067和751.42±45.50。稳定性研究表明，氯仿中的羽扇豆酚在室温下24小时内稳定（％RSD = 1.07）。从HS根和地上部分的石油醚提取物中得到的羽扇豆醇的平均回收率分别为98.66±0.65和98.77±0.39，％w / w含量（以干重计）为5.02±0.23和0.39±0.11％w / w。发现和定量的极限分别为17.85和54.11。