Forty-six strains of Trichoderma spp. have been isolated from soils in different locations and ecosystems of Algeria. They were identified at species level by analysis of their Internal Transcribed Spacers regions 1 and 2 (ITS1 and ITS2) of rDNA and a partial sequence of the Translation Elongation Factor 1-alpha (TEF 1-α) gene. The 46 Trichoderma spp. were assigned to Trichoderma atroviride (12 strains), T. gamsii (10), T. orientale (1) and 23 to T. harzianum species complex (T. harzianum, T. afroharzianum, T. atrobrunneum and T. guizhouense). In the present study we highlight that T. gamsii, T. orientale, T. atrobrunneum and T. guizhouense are being reported for the first time in Algeria. Trichoderma spp. isolates growth was evaluated at temperatures ranging from 10 to 40 °C on PDA medium. The optimum growth was recorded at 25 °C and 30 °C, and only T. orientale was able to grow at 40 °C. The in vitro test revealed the potential antagonist of Trichoderma spp. isolates against four pathogenic species associated with strategic crops in Algeria, Fusarium culmorum, Botrytis cinerea, Alternaria solani and Rhizoctonia solani. In direct confrontation, the growth rate inhibition was ranked between 37.22% and 80.95% while in indirect confrontation was between 00% and 88.89%. The biocontrol assay carried out on wheat plant showed that T. atroviride (Ta.09), T. orientale (To.15), T. afroharzianum (T af. 17 and T af. 37) and T. gamsii (T g. 39) performed well against F. culmorum the crown rot and head blight pathogen of wheat in Algeria. This finding is based on the significant decrease in disease severity compared to the control (> 82%). Data recorded, have also shown that T. atroviride Ta.09 recorded the highest percentage of disease reduction (97.28%).

木霉属的四十六株。已从阿尔及利亚不同地区和生态系统的土壤中分离出来。通过分析其rDNA的内部转录间隔区1和2（ITS1和ITS2）以及翻译延伸因子1-alpha（TEF1-α）基因的部分序列，在物种水平上鉴定了它们。46个木霉属。被分配到深绿木霉（12株），T. gamsii（10），T.东方（1）和23至哈茨木霉物种络合物（哈茨木霉，T. afroharzianum，T. atrobrunneum和T. guizhouense）。在本研究中，我们着重指出T. gamsii，T。Orientale，T. atrobrunneum和T. guizhouense被报告在阿尔及利亚的第一次。木霉属。在PDA培养基上10至40°C的温度下评估分离株的生长。最佳生长记录在25°C和30°C，只有东方锥虫能够在40°C生长。体外测试揭示了木霉菌的潜在拮抗剂。针对与阿尔及利亚的战略作物，相关的四个致病菌种株镰刀菌，灰霉病，番茄早疫病菌和纹枯病。在直接对抗中，生长抑制率在37.22％至80.95％之间，而在间接对抗中，生长抑制率在00％至88.89％之间。对小麦植物进行生物防治测定显示T.深绿（Ta.09），T.东方（To.15），T. afroharzianum（T AF 17和T AF。37）和T. gamsii（T克。 39）在阿尔及利亚，对小麦镰刀菌的冠腐病和头枯病病原体表现良好。这一发现是基于与对照相比疾病严重程度的显着降低（> 82％）。记录的数据，还表明，T.深绿Ta.09记录疾病减少（97.28％）的比例最高。