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Meiobenthic nematode Oncholaimus campylocercoides as a model in laboratory studies: selection, culture, and fluorescence microscopy after exposure to phenanthrene and chrysene
Environmental Science and Pollution Research ( IF 5.8 ) Pub Date : 2021-02-09 , DOI: 10.1007/s11356-021-12688-1
Mohamed Allouche 1 , Ahmed Nasri 1 , Abdel Halim Harrath 2 , Lamjed Mansour 2 , Saleh Alwasel 2 , Hamouda Beyrem 1 , Gabriel Plăvan 3 , Melissa Rohal-Lupher 4 , Fehmi Boufahja 1
Affiliation  

Numerous studies have focused on the response of meiofauna after exposure to polycyclic aromatic hydrocarbons (PAHs), but none has been devoted to their uptake into nematode body compartments. The present study monitored PAH uptake by Oncholaimus campylocercoides which was selected after 40 days in the laboratory through original protocols from natural sediments collected in the Old Harbor of Bizerte, Tunisia. To achieve the mono-species level, a grain size magnification was applied by gradually adding a biosubstrate made from either the crushed shells of Mytilus galloprovincialis or minced leaves of Posidonia oceanica. After selection, O. campylocercoides was cultured and fed with earthworm powder (560 mg.l-1). Thereafter, it was exposed for 3 weeks to phenanthrene and chrysene (38, 116, and 348 ppb). Fluorescence microscopy revealed higher intensities of PAHs at the spicules, mouths, and pharynges compared with the other organs considered. Moreover, the buccal fluorescence showed a significant correlation with that measured in the biosubstrate made with shells of M. galloprovincialis.



中文翻译:

灭蚊线虫Oncholaimus campylocercoides作为实验室研究的模型:暴露于菲和菊花后的选择,培养和荧光显微镜检查

许多研究都集中在暴露于多环芳烃(PAHs)之后,对动物类动物的反应,但还没有专门研究它们对线虫体腔的吸收。本研究监测了Oncholaimus campylocercoides对PAH的吸收,Oncholaimus campylocercoides是在实验室中40天后通过原始协议从突尼斯比塞大旧港收集的天然沉积物中选择的。为了达到单一物种的水平,通过逐渐添加一种生物底物来放大颗粒大小,该生物底物是由碎米氏Mytilus galloprovincialis的壳或大洋波塞冬的切碎的叶子制成的。选择后,O. campylocercoides中培养,并用蚯蚓粉末(560 mg.l馈送-1)。之后,将其暴露于菲和菲(38、116和348 ppb)3周。荧光显微镜检查显示,与所考虑的其他器官相比,在针状,口腔和咽部的PAHs强度更高。此外,颊荧光显示与在具有贝壳所作的biosubstrate测量的显著相关贻贝

更新日期:2021-02-09
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