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Molecular cloning and characterization of three CoIDA genes in Camellia oleifera
Brazilian Journal of Botany ( IF 1.6 ) Pub Date : 2021-02-09 , DOI: 10.1007/s40415-020-00691-8
Mi Yang , Xiao Hu , Xiang Ouyang , Muhammad Sajjad , Xiaoling Ma , Deyi Yuan

The INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) controls floral organ abscission in plants. IDA belongs to IDA-LIKE (IDL) gene family that is involved in regulation of Arabidopsis development. Herein, we identified three genes, CoIDA1, CoIDA2 and CoIDA3 in Camellia oleifera (Camellia oleifera Abel. cv. Huashuo) and suggested their involvement in the regulation of fruits abscission. The full-length cDNA sequences of CoIDA1, CoIDA2 and CoIDA3 were of 207 bp, 276 bp and 273 bp, encoding proteins of 68, 91 and 90 amino acids, respectively. These CoIDA genes were single exon genes (SEGs) with a conserved extended PIP motif (EPIP) at C-terminal that has been implicated to play an important role in governing protein function for enhanced flower abortion rate. The highest expression of CoIDA1 was in young peduncles and the lowest in young fruits. However, the highest expressions of CoIDA2 and CoIDA3 were both in young roots, and the lowest in young fruits. The expressions of CoIDA1 and CoIDA2 significantly increased in abscission zones (AZs) of both abnormal fruits (AF) and ethephon treated fruits (ETH-F) with respect to normal fruits (NF), which suggest that CoIDA1 and CoIDA2 genes are related to fruits abscission in C. oleifera. This study provided a preliminary understanding about CoIDA genes which could lead to their detailed functional analysis and utilization for improving C. oleifera yield potential.



中文翻译:

油茶中三个CoIDA基因的分子克隆与鉴定

花序缺陷的脱落IDA)控制在植物花器官脱落。IDA属于IDA-LIKE(IDL)基因家族,参与调节拟南芥的发育。在本文中,我们在油茶Camellia oleifera Abel。cv。Huashuo)中鉴定了三个基因CoIDA1CoIDA2CoIDA3,并暗示它们参与了对果实脱落的调控。CoIDA1CoIDA2CoIDA3的全长cDNA序列它们分别为207 bp,276 bp和273 bp,分别编码68、91和90个氨基酸。这些CoIDA基因是单外显子基因(SEG),在C端具有保守的延伸PIP基序(EPIP),这被暗示在调控蛋白质功能以提高花朵流产率方面起着重要作用。CoIDA1的最高表达是在年轻的花序梗中,最低的是在年轻的果实中。但是,CoIDA2CoIDA3的最高表达均在幼根中,而在幼果中最低。CoIDA1和CoIDA2的表达与正常水果(NF)相比,异常水果(AF)和用乙烯利处理的水果(ETH-F)的脱落区(AZ)显着增加,这表明CoIDA1CoIDA2基因与油茶中的果实脱落有关。这项研究提供了对CoIDA基因的初步了解,这可能导致它们详细的功能分析和利用来提高油茶的产量潜力。

更新日期:2021-02-09
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