Magnetic fluid hyperthermia (MFH) is a successful nanotechnology application in recent decade where a biocompatible magnetic fluid is used to kill cancer cells in a controlled heating using AC magnetic field. In the present study, two ferrite-based magnetic fluids, with and without surfactant coating, were synthesized to study the effect of the outer layer of magnetic nanoparticles on cervical cancer cells. The magnetic fluid without surfactant coating (MFWI) was made stable by providing negative charge on the surface of each particle. On the other hand, lauric acid was used as a surfactant to have a stable dispersion of particles in aqueous media (MFWL). The structural, magnetic properties and induction heating response of both the fluids were investigated using XRD, VSM, DLS, TGA, FTIR, and a high-frequency induction heater. The in vitro cytotoxicity of the synthesized fluids was observed on HeLa cells by performing MTT assay, and the effect of magnetic fluid hyperthermia was examined using Trypan blue assay. The crystallite size of surfactant stabilized particles was higher (11.0 ± 0.5 nm) compared to the charge stabilized particles (8.3 ± 0.5 nm). Induction heating experiments showed that the specific absorption rate of the surfactant-coated particles was almost double compared to ionic particle fluid. Magnetic fluid hyperthermia up to 1 hour at a concentration of 0.25 mg/mL of surfactant-coated magnetic fluid and 0.2 mg/mL concentration of charged fluid resulted in approximately 66 and 80% cell death, respectively, compared to untreated control cells. The preliminary analysis of this study shows significant cell death due to hyperthermia, wherein MFWI revealed higher cytotoxicity compared to MFWL. Additional analysis into the role of the outer stabilizing layer on nanoparticle’s surface, concentration of nanoparticles, and hyperthermic duration is desirable to utilize MFH as a futuristic anti-cancer therapeutic tool.

中文翻译：

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磁流体热疗研究磁性颗粒外层对宫颈癌细胞HeLa的影响

磁流体热疗（MFH）是近十年来成功的纳米技术应用，其中使用生物相容性磁流体在使用AC磁场的受控加热下杀死癌细胞。在本研究中，合成了两种带有和不带有表面活性剂涂层的基于铁氧体的磁性流体，以研究磁性纳米颗粒的外层对宫颈癌细胞的影响。通过在每个粒子的表面上提供负电荷，可以使没有表面活性剂涂层的磁性流体（MFWI）保持稳定。另一方面，月桂酸用作表面活性剂以使颗粒在水性介质（MFWL）中具有稳定的分散性。使用XRD，VSM，DLS，TGA，FTIR和高频感应加热器研究了两种流体的结构，磁性和感应加热响应。通过MTT法观察合成液对HeLa细胞的体外细胞毒性，并用台盼蓝法检测磁液热疗的作用。与电荷稳定的颗粒（8.3±0.5 nm）相比，表面活性剂稳定的颗粒的微晶尺寸更高（11.0±0.5 nm）。感应加热实验表明，与离子颗粒流体相比，表面活性剂涂覆的颗粒的比吸收率几乎提高了一倍。与未处理的对照细胞相比，浓度为0.25 mg / mL的表面活性剂包被的磁性流体和0.2 mg / mL的带电流体在长达1小时的磁流体热疗中分别导致大约66％和80％的细胞死亡。这项研究的初步分析显示，由于高温导致大量细胞死亡，其中MFWI显示出比MFWL更高的细胞毒性。为了将MFH用作未来的抗癌治疗工具，需要进一步分析外部稳定层在纳米颗粒表面上的作用，纳米颗粒的浓度和高温持续时间。