It was observed that the seedling biomass was correlated with final seed yield in many crops. It is helpful to dissect the genetic architecture of seedling biomass‐related traits for high yield breeding of rapeseed (Brassica napus L.). To date, the genetic architecture of seedling biomass‐related traits is still unclear in B. napus. To identify candidate genes associated with high seedling biomass, a genome‐wide association study of seedling biomass‐related traits under normal growth condition and waterlogging stress was conducted with 156 B. napus accessions. We detected six peaks including 55 single‐nucleotide polymorphisms (SNPs) significantly associated with the above‐mentioned traits in SNP association analysis and 142 UniGenes showed significantly associated with traits in gene expression marker (GEM) association analysis. Candidate genes located within the linkage disequilibrium (LD) blocks were identified, such as ADP GLUCOSE PYROPHOSPHORYLASE LARGE SUBUNIT 1 (BnaC2APL1), PS II OXYGEN‐EVOLVING COMPLEX 1 (BnaC9PSBO1), their ortholog in the Arabidopsis thaliana has been shown to play key roles in starch biosynthesis and photosynthesis. Resequencing of genomic regions revealed the non‐synonymous SNPs in coding regions may be the functional polymorphisms in the two candidate genes. Our results provide insight into the molecular mechanisms underlying the establishment of high‐seedling biomass B. napus and lay a foundation for the characterization of candidate genes aimed at developing high‐seedling B. napus varieties.

中文翻译：

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关联转录组学研究剖析了油菜籽（Brassica napus L.）幼苗生物量相关性状的遗传结构

观察到，在许多作物中，幼苗生物量与最终种子产量相关。它有利于解剖苗木生物相关性状的高产育种油菜（的遗传结构油菜L.）。迄今为止，油菜幼苗的生物量相关性状的遗传结构仍不清楚。为了确定与高幼苗生物量相关的候选基因，在156个甘蓝型油菜中进行了正常生长条件和涝渍胁迫下幼苗生物量相关性状的全基因组关联研究。加入。我们在SNP关联分析中检测到6个峰，包括55个与上述特征显着相关的单核苷酸多态性（SNP），而142个UniGenes在基因表达标记（GEM）关联分析中显示与这些特征显着相关。鉴定了位于连锁不平衡（LD）区块内的候选基因，例如ADP葡萄糖磷酸磷酸酶大亚单位1（BnaC2APL1），PS II氧气进化复合物1（BnaC9PSBO1），它们在拟南芥中的直系同源基因。已显示在淀粉生物合成和光合作用中起关键作用。基因组区域的重测序显示编码区域中的非同义SNP可能是两个候选基因中的功能多态性。我们的结果提供了对建立高幼苗生物型油菜的基础分子机制的见解，并为表征旨在发展高幼苗油菜的品种的候选基因的特性奠定了基础。