Rose (Rosa hybrida) is widely used for cut flowers and as garden plants. Stable and efficient transformation system is required for functional genomics of rose. Here, we established an efficient transformation method for rose using Agrobacterium tumefaciens-mediated transformation of embryogenic callus. Expanding rose leaves were used as explants to induce somatic embryos, which were subjected to transformation with A. tumefaciens strain GV3101 using Green Fluorescence Protein (GFP) as a marker gene. It took about 8 months to generate transgenic shoots from embryogenic callus. PCR, RT-PCR, Southern and Western blotting, as well as stereoscopic fluorescence microscopy analysis demonstrated that GFP transgenes integrated stably into the rose genome. According to our data, a transformation efficiency of up to 6% can be achieved by following this optimized protocol.

玫瑰（Rosa hybrida）广泛用于切花和园林植物。玫瑰功能基因组学需要稳定高效的转化系统。在这里，我们使用根癌农杆菌介导的胚性愈伤组织转化建立了一种有效的玫瑰转化方法。以扩大的玫瑰叶为外植体诱导体细胞胚，以绿色荧光蛋白( GFP )为标记基因，用根癌农杆菌GV3101菌株进行转化。从胚性愈伤组织产生转基因枝条需要大约 8 个月的时间。PCR、RT-PCR、Southern 和 Western 印迹以及立体荧光显微镜分析表明，GFP转基因稳定整合到玫瑰基因组中。根据我们的数据，按照这个优化的协议可以实现高达 6% 的转换效率。