Abstract

Bacterial strains ANT 17 and ANT 56, antagonistic to plant pathogenic fungi Bipolaris sorokiniana, were isolated from activated sludge. Physiological, biochemical, and culture morphological properties and analysis of the 16S rRNA gene sequence and composition of fatty acids of cell walls of strains ANT 17 and ANT 56 supported its classification as the species Pseudomonas laurentiana. It was shown that strains P. laurentiana ANT 17 and P. laurentiana ANT 56 possess a set of properties characteristic of PGP (plant growth-promoting) microorganisms: they exhibit antifungal activity against phytopathogenic micromycetes and are capable of decomposing phosphates and synthesizing phytohormonal substances. Inoculation of cucumber, tomato, and cabbage seeds had a beneficial effect on their germination. Presowing treatment of wheat seeds under conditions of a natural infectious background with an inoculum of the isolated bacterial strains contributed to a decrease in the spread of fungi that cause root rot. The possibility of using strains P. laurentiana ANT 17 and P. laurentiana ANT 56 in biotechnology in order to increase the productivity of agroecosystems is suggested. The ability to stimulate the growth and development of plants for P. laurentiana strains is shown for the first time.

中文翻译：

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劳氏假单胞菌的新细菌菌株：农业生物技术的有前途的代理

摘要

从活性污泥中分离出与植物病原性真菌Bipolaris sorokiniana拮抗的细菌菌株ANT 17和ANT 56 。生理，生化和培养形态学特性以及对ANT 17和ANT 56菌株细胞壁脂肪酸16S rRNA基因序列和脂肪酸组成的分析支持其分类为劳氏假单胞菌（Pseudomonas laurentiana）物种。结果表明，劳氏假单胞菌ANT 17和劳氏假单胞菌ANT 56具有一组PGP（促进植物生长）微生物的特性：它们对植物病原性微真菌显示出抗真菌活性，并且能够分解磷酸盐和合成植物激素物质。接种黄瓜，番茄和卷心菜种子对其发芽具有有益作用。在自然感染背景下用分离的细菌菌株接种物对小麦种子进行播前处理，有助于减少引起根腐的真菌传播。建议在生物技术中使用劳氏假单胞菌ANT 17和劳氏假单胞菌ANT 56菌株以提高农业生态系统的生产力。刺激植物生长和发育的能力首次显示了劳氏疟原虫菌株。