Journal of Cluster Science ( IF 2.8 ) Pub Date : 2021-02-08 , DOI: 10.1007/s10876-021-02004-9 Jamaan S. Ajarem , Saleh N. Maodaa , Ahmed A. Allam , Mohamed M. Taher , Mohamed Khalaf
Eco-friendly synthesis of Cobalt oxide nanoparticles (Co3O4NPs) was assessed by the aqueous extract of red algae and their utilization as cytotoxicity, antioxidant, anticoagulant, antibacterial, and anti-cancer properties. UV–vis, SEM, TEM, FTIR, EDAX, XRD, and TGA are tools used for characterization purposes. The size of the Co3O4NPs crystal of 28.2 ± 7.8 nm was measured based on the X-ray diffraction analysis. Biosynthesized Co3O4NPs showed uniform spherical morphology with an average diameter of 29.8 ± 8.6 nm according to TEM and SEM images. Furthermore, Co3O4NPs biological properties such as determination of the antibacterial properties of Co3O4NPs by the zone of inhibition method and the minimum inhibitory concentration (MIC) were determined using a dilution broth agar test. Co3O4NPs indicated higher antibacterial than oxytetracycline standard antibiotics. On the other hand, a scavenging free radical DPPH study was done to determine the propensity for antioxidants that showed a similar capacity for antioxidants in Co3O4NPs. The biosynthesized Co3O4NPs are effective erythrocyte viability dose-dependently and indicated this method was nontoxic. Moreover, bioinspired Co3O4NPs has promising results against HepG2 cancer cells (IC50: 41.4 μg/ml). Most notably, biogenic Co3O4NPs have anticoagulant and thrombolytic activities by the addition of nanoparticles to human blood samples under practical conditions. According to these context findings, Co3O4NPs would be used as a medicinal aid along with its anti-cytotoxic, antioxidant, and effects on bacteria, and cancer.
中文翻译:
含红藻提取物的氧化钴纳米粒子的良性合成:抗氧化,抗微生物,抗癌和抗凝血活性
通过红藻的水提取物及其作为细胞毒性,抗氧化剂,抗凝剂,抗菌和抗癌特性的应用,评估了氧化钴纳米颗粒(Co 3 O 4 NPs)的环保合成。UV-vis,SEM,TEM,FTIR,EDAX,XRD和TGA是用于表征的工具。基于X射线衍射分析,测得Co 2 O 4 NPs晶体的尺寸为28.2±7.8nm。根据TEM和SEM图像,生物合成的Co 3 O 4 NPs具有均匀的球形形态,平均直径为29.8±8.6 nm。此外,Co 3 O 4使用稀释肉汤琼脂试验确定NPs的生物学特性,例如通过抑制区确定Co 3 O 4 NPs的抗菌特性和最小抑制浓度(MIC)。Co 3 O 4 NPs比土霉素标准抗生素具有更高的抗菌性。另一方面,进行了清除自由基DPPH的研究,以确定抗氧化剂的倾向性,该倾向表明Co 3 O 4 NP中的抗氧化剂能力相似。生物合成的Co 3 O 4 NPs具有剂量依赖性的有效红细胞生存能力,表明该方法无毒。此外,受生物启发的Co 3O 4 NPs对HepG2癌细胞有希望的结果(IC50:41.4μg/ ml)。最值得注意的是,通过在实际条件下向人体血液样本中添加纳米颗粒,生物型Co 3 O 4 NP具有抗凝血和溶栓活性。根据这些背景发现,Co 3 O 4 NP连同其抗细胞毒性,抗氧化剂以及对细菌和癌症的作用将被用作药物辅助。