Purpose This study was designed to investigate the biomechanical properties of nonirradiated (NI) and irradiated (IR) peroneus tendons to determine if they would be suitable allografts, in regards to biomechanical properties, for anterior cruciate ligament reconstruction after a dose of 1.5–2.5 Mrad. Methods Seven pairs of peroneus longus (PL) and ten pairs of peroneus brevis (PB) tendons were procured from human cadavers. The diameter of each allograft was measured. The left side of each allograft was IR at 1.5–2.5 Mrad, whereas the right side was kept aseptic and NI. The allografts were thawed, kept wet with saline, and attached in a single-strand fashion to custom freeze grips using liquid nitrogen. A preload of 10 N was then applied and, after it had reached steady state, the allografts were pulled at 4 cm/sec. The parameters recorded were the displacement and force. Results The elongation at the peak load was 10.3±2.3 mm for the PB NI side and 13.5±3.3 mm for the PB IR side. The elongation at the peak load was 17.4±5.3 mm for the PL NI side and 16.3±2.0 mm for the PL IR side. For PL, the ultimate load was 2,091.6±148.7 N for NI and 2,122.8±380.0 N for IR. The ultimate load for the PB tendons was 1,485.7±209.3 N for NI and 1,318.4±296.9 N for the IR group. The ultimate stress calculations for PL were 90.3±11.3 MPa for NI and 94.8±21.0 MPa for IR. For the PB, the ultimate stress was 82.4±19.0 MPa for NI and 72.5±16.6 MPa for the IR group. The structural stiffness was 216.1±59.0 N/mm for the NI PL and 195.7±51.4 N/mm for the IR side. None of these measures were significantly different between the NI and IR groups. The structural stiffness was 232.1±45.7 N/mm for the NI PB and 161.9±74.0 N/mm for the IR side, and this was the only statistically significant difference found in this study (P=0.034). Conclusion Our statistical comparisons found no significant differences in terms of elongation, ultimate load, or ultimate stress between IR and NI PB and PL tendons. Only the PB structural stiffness was affected by irradiation. Thus, sterilizing allografts at 1.5–2.5 Mrad of gamma irradiation does not cause major alterations in the tendons’ biomechanical properties while still providing a suitable amount of sterilization for anterior cruciate ligament reconstruction.

中文翻译：

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伽马射线辐照对腓骨肌腱生物力学特性的影响

目的本研究旨在研究非辐照（NI）和辐照（IR）腓骨肌腱的生物力学特性，以确定它们是否适合用于同种异体骨移植，考虑到生物力学特性，在剂量为1.5–2.5 Mrad后进行前交叉韧带重建。方法从人体尸体上采购7对腓骨长肌（PL）和10对腓骨短肌（PB）肌腱。测量每个同种异体移植物的直径。每个同种异体移植的左侧在1.5-2.5 Mrad处发生IR，而右侧则保持无菌和NI。解冻同种异体移植物，用盐水保持湿润，并使用液氮以单链方式附着到定制的冷冻夹上。然后施加10 N的预载荷，并在达到稳定状态后，以4 cm / sec的速度移植同种异体移植物。记录的参数是位移和力。结果PB NI侧的峰值载荷伸长率为10.3±2.3 mm，PB IR侧的峰值载荷伸长率为13.5±3.3 mm。PL NI侧的峰值载荷伸长率为17.4±5.3 mm，PL IR侧的峰值伸长率为16.3±2.0 mm。对于PL，NI的极限载荷为2,091.6±148.7 N，IR的极限载荷为2,122.8±380.0N。PB肌腱的最终载荷对于NI为1,485.7±209.3 N，对于IR组为1,318.4±296.9N。PL的极限应力计算对于NI为90.3±11.3 MPa，对于IR为94.8±21.0 MPa。对于PB，NI的极限应力为82.4±19.0 MPa，IR组的极限应力为72.5±16.6 MPa。NI PL的结构刚度为216.1±59.0 N / mm，IR侧的结构刚度为195.7±51.4 N / mm。NI和IR组之间的这些测量均无显着差异。结构刚度为232.1±45。NI PB为7 N / mm，IR侧为161.9±74.0 N / mm，这是本研究中唯一具有统计学意义的差异（P = 0.034）。结论我们的统计比较发现，IR，NI PB和PL肌腱在伸长率，极限载荷或极限应力方面无显着差异。仅PB结构刚度受辐射影响。因此，在1.5至2.5 Mrad的伽马射线辐照下对同种异体移植物进行灭菌不会导致肌腱的生物力学特性发生重大变化，同时仍可为前交叉韧带重建提供适当的灭菌量。IR，NI PB和PL肌腱之间的极限载荷或极限应力。仅PB结构刚度受辐射影响。因此，在1.5至2.5 Mrad的伽马射线辐照下对同种异体移植物进行灭菌不会导致肌腱的生物力学特性发生重大变化，同时仍可为前交叉韧带重建提供适当的灭菌量。IR，NI PB和PL肌腱之间的极限载荷或极限应力。仅PB结构刚度受辐射影响。因此，在1.5至2.5 Mrad的伽马射线辐照下对同种异体移植物进行灭菌不会导致肌腱的生物力学特性发生重大变化，同时仍可为前交叉韧带重建提供适当的灭菌量。