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Morphological and molecular identification of four Purpureocillium isolates and evaluating their efficacy against the sweet potato whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae)
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2021-02-07 , DOI: 10.1186/s41938-021-00372-y Tingfei Sun , Jianhui Wu , Shaukat Ali
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2021-02-07 , DOI: 10.1186/s41938-021-00372-y Tingfei Sun , Jianhui Wu , Shaukat Ali
Entomopathogenic fungi are widely distributed and well described within the fungal kingdom. This study reports the isolation, characterization, and virulence of 4 Purpureocillium lilacinum isolates against the sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Four strains of Purpureocillium lilacinum (XI-1, XI-4, XI-5, and J27) were isolated from soil samples from different localities of China. The morphological studies observed that four strains showed essentially the same morphological characteristics. After 7 days of cultivation, the colonies were purple, round, and bulged. Conidia were single-celled, oval to spindle-shaped, chain-like, and the spore size was about 2.0–2.3 × 3.1–4.0 μm. The genome-based identification results showed that ITS sequences of XI-1 (GenBank accession # MW386433), XI-4 (GenBank accession # MW386434), XI-5 (GenBank accession # MW386435), and J27 (GenBank accession # MW386436) were similar to another P. lilacinum. The newly identified strains of P. lilacinum proved pathogenicity to B. tabaci under laboratory conditions. In addition, the P. lilacinum isolate XI-5 was the most virulent one against different nymphal instars of whitefly having median lethal concentration (LC50) values of 4.99 × 106, 4.82 × 105, and 2.85 × 106 conidia/ml, respectively, 7 days post application. The newly isolated strains of P. lilacinum can be developed as a potential biopesticide against the whitefly although extensive field bioassays as well as development of proper formulation are still required.
更新日期：2021-02-08
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