Planktonic communities from Juan de Nova lagoon were submitted to a single exposure of diuron or naphthalene in triplicate outdoor 40-L mesocosms for 5 days. The study followed the temporal changes of the assemblages by determining abundances and taxonomic level of microbial (heterotrophic prokaryotes, picocyanobacteria, heterotrophic protists and phytoplankton) and metazooplankton communities. Exposure to both contaminants did not result in significant changes in phytoplankton abundances. Microscopic identification demonstrated the dominance of Bacillariophyta (mostly Chaetoceros tenuissimus) accounting for more than 95% of the microalgae detected in all mesocosms. Using 18S rRNA metabarcoding, temporal changes in the proportion of eukaryotic operational taxonomic units (OTUs) were highlighted for most taxonomic groups. Diuron had no significant effect on the abundance of eukaryotic picoheterotrophs, ciliates, whereas naphthalene had a significant positive effect on heterotrophic prokaryotes. Metazooplankton was not significantly modified under contaminant exposure, except for copepods nauplii in presence of diuron. Multivariate analysis indicated significant responses of communities linked to the experimental time and conditions. The analysis also revealed a modification of the trophic relationships among communities over time, from a dominant multivorous food web after 48 h towards a reduced microbial loop after 120 h of experiment time.

来自Juan de Nova泻湖的浮游生物群落在三重室外40-L的中观环境中暴露于敌草隆或萘中5次。该研究通过确定微生物的丰度和生物分类水平（异养原核生物，微蓝细菌，异养原生物和浮游植物）和后生浮游生物群落来追踪这些组合的时间变化。两种污染物的暴露都不会导致浮游植物丰度的显着变化。显微镜鉴定表明，杆菌属（多数为Chaetoceros tenuissimus）占主导地位，占所有中观范围内检测到的微藻的95％以上。使用18SrRNA metabarcoding，对于大多数分类学组而言，真核生物操作分类学单位（OTUs）的比例的时间变化是突出的。敌草隆对丰富的真核微异养生物，纤毛虫没有显着影响，而萘对异养原核生物具有显着的正作用。除了暴露于敌草隆的co足无节幼体外，在污染物暴露下，元祖浮游生物没有显着改变。多变量分析表明，与实验时间和条件有关的群落反应显着。分析还显示，随着时间的推移，各社区之间的营养关系发生了变化，从48小时后的主要杂食性食物网到120小时实验时间后的微生物循环减少。