To show a spectrum of histone H1 subtypes (H1.1-H1.5) activity realized through the protein-protein interactions, data selected from APID resources were processed with sequence-based bioinformatics approaches. Histone H1 subtypes participate in over half a thousand interactions with nuclear and cytosolic proteins (ComPPI database) engaged in the enzymatic activity and binding of nucleic acids and proteins (SIFTER tool). Small-scale networks of H1 subtypes (STRING network) have similar topological parameters (P > .05) which are, however, different for networks hubs between subtype H1.1 and H1.4 and subtype H1.3 and H1.5 (P < .05) (Cytoscape software). Based on enriched GO terms (g:Profiler toolset) of interacting proteins, molecular function and biological process of networks hubs is related to RNA binding and ribosome biogenesis (subtype H1.1 and H1.4), cell cycle and cell division (subtype H1.3 and H1.5) and protein ubiquitination and degradation (subtype H1.2). The residue propensity (BIPSPI predictor) and secondary structures of interacting surfaces (GOR algorithm) as well as a value of equilibrium dissociation constant (ISLAND predictor) indicate that a type of H1 subtypes interactions is transient in term of the stability and medium-strong in relation to the strength of binding. Histone H1 subtypes bind interacting partners in the intrinsic disorder-dependent mode (FoldIndex, PrDOS predictor), according to the coupled folding and binding and mutual synergistic folding mechanism. These results evidence that multifunctional H1 subtypes operate via protein interactions in the networks of crucial cellular processes and, therefore, confirm a new histone H1 paradigm relating to its functioning in the protein-protein interaction networks.

中文翻译：

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人类组蛋白 H1 亚型相互作用网络的调查：对组蛋白 H1 功能的影响

为了显示通过蛋白质-蛋白质相互作用实现的一系列组蛋白 H1 亚型（H1.1-H1.5）活性，从 APID 资源中选择的数据使用基于序列的生物信息学方法进行处理。组蛋白 H1 亚型参与了与参与酶活性和核酸和蛋白质结合（SIFTER 工具）的核和细胞质蛋白（ComPPI 数据库）的五千多种相互作用。H1 亚型的小规模网络（STRING 网络）具有相似的拓扑参数（P  > .05），但是对于亚型 H1.1 和 H1.4 以及亚型 H1.3 和 H1.5 之间的网络枢纽，这些拓扑参数不同（P < .05)（Cytoscape 软件）。基于相互作用蛋白的丰富 GO 术语（g:Profiler 工具集），网络枢纽的分子功能和生物过程与 RNA 结合和核糖体生物发生（亚型 H1.1 和 H1.4）、细胞周期和细胞分裂（亚型 H1 .3 和 H1.5）和蛋白质泛素化和降解（亚型 H1.2）。残留倾向（BIPSPI 预测因子）和相互作用表面的二级结构（GOR 算法）以及平衡解离常数值（ISLAND 预测因子）表明，一类 H1 亚型相互作用在稳定性方面是短暂的，中强与结合强度的关系。组蛋白 H1 亚型以内在疾病依赖模式（FoldIndex，PrDOS 预测因子）结合相互作用的伙伴，根据耦合折叠和绑定和相互协同折叠机制。这些结果证明多功能 H1 亚型通过关键细胞过程网络中的蛋白质相互作用起作用，因此证实了与其在蛋白质-蛋白质相互作用网络中的功能相关的新组蛋白 H1 范式。