Malonyl-CoA synthetases may modulate cell responses to abiotic stress by regulating stress-related signaling transduction pathways or activating expression of transcription factors. However, the molecular mechanism of cold stress tolerance enhanced by malonyl-CoA synthetase is not fully understood. Here, we report that overexpression of the Arabidopsis thaliana malonyl-CoA synthetase gene AAE13.1 resulted in increased cell viability and growth rate and decreased thiobarbituric acid reactive substances under cold stress in rice (Oryza sativa L.), tobacco (Nicotiana tabacum), and slash pine (Pinus elliottii Engelm.). AAE13.1 was associated with cold stress tolerance by increasing the activity of ascorbate peroxidase, catalase, polyphenol oxidase, and peroxidase and the accumulation of acid phosphatase and alkaline phosphatase. Among six rice mitogen-activated protein kinase (MAPK) genes examined, AAE13.1 overexpression increased the expression of OsMAPK genes during cold stress. AAE13.1 activated expression of stress-response genes OsMAPK1, OsMAPK2, and OsMAPK3, indicating that AAE13.1 enhances cold stress tolerance by regulating expression of MAPK genes in plant cells. These results increase our understanding of cold stress tolerance in species of monocotyledons, dicotyledons, and gymnosperms.

丙二酰辅酶A合成酶可通过调节应激相关的信号转导途径或激活转录因子的表达来调节细胞对非生物胁迫的反应。然而，通过丙二酰辅酶A合成酶增强的耐冷胁迫的分子机理尚不完全清楚。在这里，我们报告的过表达拟南芥丙二酰-CoA合成酶基因AAE13.1导致增加的细胞生存力和生长速率和水稻（减小低温胁迫下硫代巴比土酸反应物质稻L.），烟草（烟草），和斜线松（Pinus elliottii Engelm。）。AAE13.1通过增加抗坏血酸过氧化物酶，过氧化氢酶，多酚氧化酶和过氧化物酶的活性以及酸性磷酸酶和碱性磷酸酶的积累，与耐寒性有关。在检查的六个水稻有丝分裂原激活蛋白激酶（MAPK）基因中，AAE13.1的过表达增加了冷胁迫期间OsMAPK基因的表达。AAE13.1激活了应激反应基因OsMAPK1，OsMAPK2和OsMAPK3的表达，表明AAE13.1通过调节MAPK的表达增强了寒冷胁迫的耐受性植物细胞中的基因。这些结果增加了我们对单子叶植物，双子叶植物和裸子植物物种的耐冷胁迫的理解。