In this paper, the serpentine-shaped semi-packed chromatographic columns embedded with the micro-post array were developed to separate the characteristic markers of advanced fibrosis. The 3 m long micro separation columns with a rectangular channel cross-sectional dimension of 250 μm 200 μm and the micropost diameter of 30 μm were prepared by the micro-electromechanical system (MEMS) technology. Based on the static coating process, two kinds of microcolumns (Sp1 and Sp2) with different stationary phase coatings were prepared for comparison. In detail, the Sp1 column was only coated with polydimethylsiloxane (PDMS), and the Sp2 column was coated with PDMS functionalized by graphene oxide quantum dots (GOQDs). GOQDs is a carbon-based nanomaterial that had similar properties to graphene oxide (GO). Moreover, the microcolumns are connected to a conventional gas chromatograph for testing. Alkanes (C₅–C₁₄) and benzene series (benzene, toluene, o-xylene, 1,3,5- trimethylbenzene) were used to evaluate the separation characteristics of microcolumns, and the effect of constant column temperature and heating rate on chromatographic performance was studied. The experimental results show that the potential characteristic markers of advanced fibrosis (pentane and benzene) could be effectively separated in alkanes and benzene series, respectively, and the separation effect of Sp2 was better than that of Sp1. In addition, the Sp2 column also showed good repeatability. Therefore, the GOQDs-functionalized PDMS coated column could assist the microdetector of the microsystem under development to rapidly, accurately and effectively distinguish the markers, and is expected to become a candidate for micro gas chromatography (μGC) column for micro-system integration.

本文开发了嵌有微柱阵列的蛇形半填充色谱柱，以分离晚期纤维化的特征标记。用的矩形通道的横截面尺寸3米长的微分离柱250 μ米200 μ m和30的直径微柱μm由微机电系统（MEMS）技术制备。在静态涂覆过程的基础上，制备了两种具有不同固定相涂层的微柱（Sp1和Sp2）进行比较。详细地，Sp1柱仅涂覆有聚二甲基硅氧烷（PDMS），而Sp2柱仅涂覆有由氧化石墨烯量子点（GOQD）功能化的PDMS。GOQD是一种碳基纳米材料，具有与氧化石墨烯（GO）相似的特性。此外，将微柱连接到常规气相色谱仪上进行测试。烷烃（C ₅ –C ₁₄）和苯系列（苯，甲苯，邻二甲苯，1,3,5-三甲基苯）用于评估微柱的分离特性，并研究了恒定柱温和加热速率对色谱性能的影响。实验结果表明，可以在烷烃和苯系列中分别有效分离潜在的晚期纤维化标志物（戊烷和苯），Sp2的分离效果优于Sp1。此外，Sp2色谱柱也显示出良好的重复性。因此，GOQDs功能化的PDMS涂层色谱柱可以帮助正在开发的微系统的微检测器快速，准确和有效地区分标记，并有望成为微气相色谱的候选者（μGC）列，用于微系统集成。