Abstract

Elymus purpuraristatus and Roegneria calcicola are perennial species of wheatgrass in Triticeae (Poaceae), treated as members of different genera on the basis of morphological characteristics. However, their genomic constitution is still unknown and their taxonomic status remains disputed. To identify their genomic constitution, we performed chromosome pairing analysis, genomic in situ hybridization and phylogenetic analyses on E. purpuraristatus and R. calcicola. The meiotic pairing results for the artificial hybrids of E. wawawaiensis (StH) × E. purpuraristatus, E. purpuraristatus × Campeiostachys dahurica var. tangutorum (StYH) and R. calcicola × C. kamoji (StYH) suggested that E. purpuraristatus and R. calcicola are allohexaploid with the St, Y and H genomes. The genomic in situ hybridization results of E. purpuraristatus and R. calcicola confirmed that they possess two sets of the H genome and two sets of the StY genome. Phylogenetic analyses suggested that Acc1 and DMC1 sequences from E. purpuraristatus and R. calcicola grouped with the St genome clade, Y genome clade and H genome clade, respectively. In summary, cytological and phylogenetic results indicated that the genome formula of E. purpuraristatus and R. calcicola is StStYYHH. According to the genomic system of classification in Triticeae, E. purpuraristatus and R. calcicola should be classified in Campeiostachys as C. purpuraristata and C. calcicola, respectively.

中文翻译：

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基于细胞遗传学和系统发育分析的紫草和卷心菜（禾本科：Triticeae）的基因组组成和分类学修订

摘要

Elymus purpuraristatusus和Roegneria calcicola是小麦属（禾本科）的多年生小麦草，根据形态特征被视为不同属的成员。然而，它们的基因组组成仍是未知的，并且它们的分类学地位仍然是有争议的。为了鉴定它们的基因组组成，我们对紫癜和R. calcicola进行了染色体配对分析，基因组原位杂交和系统发育分析。wawawaiensis（StH）×  purpuraristatusus，purpurariaristatus×Campeiostachys dahurica var人工杂交的减数分裂配对结果。tangutorum（StYH）和R. calcicola  ×  C. kamoji（StYH）表明E. purpuraristatusus和R. calcicola是具有St，Y和H基因组的异六倍体。E. purpuraristatusus和R. calcicola的基因组原位杂交结果证实，它们拥有两组H基因组和StY基因组。系统发育分析表明，ACC1和DMC1从序列E. purpuraristatusR. calcicola和R. calcicola分别与St基因组进化枝，Y基因组进化枝和H基因组进化枝分组。总之，细胞学和系统发育的结果表明，基因组式E. purpuraristatus和R.灰岩是StStYYHH。根据小麦的分类基因组系统，紫菜中的E. purpuraristatus和灰熊中的R. calcicola在Campeiostachys中应分别分类为C. purpuraristata和C. calcicola。