Downstream processing of soluble recombinant proteins from Escherichia coli is complicated by the need to access the intracellular product by cell disruption and to separate the target protein from impurities, particularly host cell protein, DNA, endotoxins and lipids. We previously demonstrated the ability of the E. coli X‐press strain to leak high amounts of product to the culture medium without sacrificing viability. In this case study, we assessed the economic and ecological benefit of this strain for downstream processing in direct comparison to the industrial standard E. coli BL21(DE3). Staphylococcal Protein A was used as a model protein. We performed recombinant protein production, primary recovery and capture by anion exchange chromatography at laboratory scale, and used the data obtained for estimating costs and resource consumption by economic modeling.

中文翻译：

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泄漏的大肠杆菌菌株用于下游加工的经济和生态效益：金黄色葡萄球菌蛋白A的案例研究

来自大肠杆菌的可溶性重组蛋白的下游加工由于需要通过细胞破坏来进入细胞内产物并使靶蛋白与杂质，特别是宿主细胞蛋白，DNA，内毒素和脂质分离而变得复杂。我们先前证明了E.coli X-press菌株能够在不牺牲活力的情况下将大量产物泄漏到培养基中。在本案例研究中，我们直接与工业标准大肠杆菌进行了比较，评估了该菌株在下游加工中的经济和生态效益。BL21（DE3）。葡萄球菌蛋白A用作模型蛋白。我们在实验室规模上通过阴离子交换色谱进行了重组蛋白的生产，初步回收和捕获，并使用所获得的数据通过经济建模来估算成本和资源消耗。