Background

Coronary artery disease (CAD) and stroke are major causes of cardiovascular diseases related deaths. Conventional risk factors cannot explain the changes in atherosclerosis. New and useful diagnostic markers are required. MicroRNAs are small, noncoding RNA that regulate the gene expression implicated in the pathogenesis of various cardiovascular diseases. Endothelial dysfunction is involved in the early event of the atherosclerosis process.

Aims

The current study was designed to evaluate the vascular endothelium-enriched miRNAs would be altered in CAD patients.

Methods

Circulating miR-126 & 122 levels were measured in serum from 78 CAD patients and 60 non CDA patients by qRT-PCR analysis.

Results

MiR-122 was significantly down regulated in CAD patients (p = 0.001), however the level of miR-126 did not show any change (p = 0.507). Remarkably, the level of miR-126 was significantly decreased in patients with CAD and high small dense low density lipoprotein (sdLDL) level. The level of miR-126 was significantly increased when sdLDL was higher in patients with risk factors for CAD but did not have angiographically significant CAD.

Conclusion

. In CAD patient’s, miR-126 level was lowered compared to non CAD patients, however the difference was not significant (0.507). However we found a direct relationship between endothelium-enriched miR-126 and sdLDL in patients with or without CAD. Our finding suggests that miR-126 may have a potential role in sdLDL cholesterol metabolism. Mir-122 plays a role in cholesterol biosynthesis and deteriorates the cardiovascular system through the process of inflammation, apoptosis, oxidative stress and ECM deposition in a number of cardiovascular diseases.

中文翻译：

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冠状动脉疾病患者的循环 microRNA-126 & 122：与小而密 LDL 的相关性

背景

冠状动脉疾病 (CAD) 和中风是心血管疾病相关死亡的主要原因。传统的危险因素不能解释动脉粥样硬化的变化。需要新的和有用的诊断标志物。MicroRNA 是小的非编码 RNA，可调节与各种心血管疾病发病机制有关的基因表达。动脉粥样硬化过程的早期事件涉及内皮功能障碍。

宗旨

目前的研究旨在评估富含血管内皮的 miRNA 在 CAD 患者中会发生改变。

方法

通过 qRT-PCR 分析测量了来自 78 名 CAD 患者和 60 名非 CDA 患者的血清中的循环 miR-126 和 122 水平。

结果

MiR-122 在 CAD 患者中显着下调 (p = 0.001)，但 miR-126 的水平没有显示任何变化 (p = 0.507)。值得注意的是，冠心病患者的miR-126水平显着降低，小而密低密度脂蛋白（sdLDL）水平高。当有 CAD 危险因素但没有血管造影显着 CAD 的患者中 sdLDL 较高时，miR-126 的水平显着增加。

结论

. 在 CAD 患者中，与非 CAD 患者相比，miR-126 水平降低，但差异不显着（0.507）。然而，我们发现在患有或不患有 CAD 的患者中富含内皮的 miR-126 和 sdLDL 之间存在直接关系。我们的发现表明 miR-126 可能在 sdLDL 胆固醇代谢中具有潜在作用。Mir-122 在胆固醇生物合成中发挥作用，并通过炎症、细胞凋亡、氧化应激和 ECM 沉积过程在许多心血管疾病中恶化心血管系统。