The study aimed at determining the effects of Zn sources, used with potato fiber (PF) or lignocellulose (LC), on microbiota ecology in the large intestine of pigs. The hypothesis was that organic Zn source, i.e. Zn glycinate (ZnGly), and PF improve polysaccharide digestibility via effects on microbiota involved in degradation of dietary fiber. Twenty four piglets of initial body weight 10.8 ± 0.82 kg were divided into 4 groups (n=6) fed diets with: LC and ZnSO₄, LC and ZnGly, PF and ZnSO₄, and PF and ZnGly. Fiber supplements introduced 10 g crude fiber/kg diet, while Zn additives provided 120 mg Zn/kg diet. After 4 weeks of feeding digesta was taken from 4 segments of the large intestine. Pigs fed ZnGly diets had greater population of Clostridium herbivorans in the cecum (P = 0.021) and proximal colon (P = 0.024), and reduced that of Clostridium spp. in the proximal colon (P = 0.037) in comparison with ZnSO₄. ZnGly had no effect on bacterial enzymes activity but increased total phenols concentration in the proximal (P = 0.012) and distal colon (P = 0.008). Feeding PF diets decreased Clostridium spp. population in the distal colon (P = 0.010), while increased it in the proximal (P < 0.001) colon (P = 0.034) as compared to LC diets. In all parts of the colon, pigs fed PF diets had β-glucosidase activity twice as high as animals on LC diets (P < 0.05), while activity of cellulase did not differ between treatments. The interaction affected Clostridium spp. population in the cecum (P = 0.034) and middle colon (P = 0.005), ammonia concentration (P = 0.032) and β-glucosidase activity (P = 0.026) in the proximal colon, and isoacid concentrations in the distal colon (P < 0.05). The lack of interactive effect on most of the analyzed indices suggest that Zn source and fiber supplementation act independently on microbiota ecology and ZnGly and PF contribute to increase of fiber digestibility in different ways, i.e. by stimulation of C. herbivorans growth or by increase of β-glucosidase activity.

该研究旨在确定与马铃薯纤维（PF）或木质纤维素（LC）一起使用的锌源对猪大肠微生物群生态的影响。假设是有机锌源，即甘氨酸锌（ZnGly）和PF通过对涉及膳食纤维降解的微生物群的影响来提高多糖消化率。将24只初始体重为10.8±0.82 kg的仔猪分为4组（n = 6），分别饲喂LC和ZnSO ₄，LC和ZnGly，PF和ZnSO ₄以及PF和ZnGly。膳食纤维补充剂每公斤日粮添加10克粗纤维，而锌添加剂提供每公斤日粮120毫克锌。喂养4周后，从大肠的4个部分中提取消化物。饲喂ZnGly日粮的猪的食肉梭状芽孢杆菌种群更多在盲肠（P  = 0.021）和近端结肠（P  = 0.024）中，Clostridium spp减少。 与ZnSO ₄相比，在近端结肠中的P＜0.037 。ZnGly对细菌的酶的活性没有影响，但在近端（增加的总酚浓度P  = 0.012）和远端结肠（P  = 0.008）。饲喂PF日粮可减少梭状芽胞杆菌。远端结肠的数量（P  = 0.010），而近端结肠的数量增加（P <0.001）（P = 0.034）。在结肠的所有部位，饲喂PF日粮的猪的β-葡萄糖苷酶活性是LC日粮的两倍（P <0.05），而纤维素酶的活性在两种处理之间没有差异。相互作用影响梭状芽胞杆菌。盲肠（P  = 0.034）和结肠中部（P  = 0.005）， 近端结肠中的氨浓度（P  = 0.032）和β-葡萄糖苷酶活性（P = 0.026）以及远端结肠中的异酸浓度（P<0.05）。缺乏对大多数分析指标的交互作用，这表明锌源和纤维的添加独立地作用于微生物群落生态，而ZnGly和PF则以不同方式（例如通过刺激草食梭菌的生长或通过增加β）促进纤维消化率的提高。 -葡糖苷酶活性。