In this work, the extractive potential, for biomolecules, of several aqueous biphasic systems (ABS) composed of polyethylene glycol (PEG) with varying molecular weight (400 and 4000) and different biodegradable organic salts, sodium citrate (Na₃C₆H₅O₇), sodium tartrate (Na₂C₄H₄O₆), and sodium succinate (Na₂C₄H₄O₄), were evaluated in the presence of the ionic liquid (IL) 1-butyl-3-methylimidazolium bromide ([C₄C₁im]Br) as adjuvant. For this purpose, firstly, the binodal curves and the liquid-liquid equilibrium (LLE) data of the studied ABS were determined, at T = 298.15 K, by the addition of 5 wt % IL to investigate its effect on the two-phase formation. The tie-line lengths (TLL) were also addressed. To evaluate the extraction capacity of the studied ABS for biomolecules, the essential amino acid L-tryptophan (Trp) was used as a molecular probe. Thus, the partition coefficients of Trp, K_Trp, were determined, at T = 298.15 K, in ABS with and without IL. The effects of salt, PEG molecular weight, and ABS composition on the partition coefficients of Trp were also investigated. It was found that the K_Trp values are increased with increasing the salting-out strength of salts, PEG molecular weight, and the ABS composition. Moreover, in the studied ABS, by the addition of 5 wt % [C₄C₁im]Br into the ABS containing PEG 400, the K_Trp values are increased, whereas, the opposite is observed for systems with PEG 4000. Finally, the experimental LLE and partition coefficients data are accurately correlated using the NRTL model.

在这项工作中，几种生物分子的萃取潜能由具有不同分子量（400和4000）的聚乙二醇（PEG）和不同的可生物降解的有机盐，柠檬酸钠（Na ₃ C ₆ H ₅）组成的水性双相系统（ABS）。在离子液体（IL）1-丁基-3-甲基咪唑鎓盐的存在下评估O ₇），酒石酸钠（Na ₂ C ₄ H ₄ O ₆）和琥珀酸钠（Na ₂ C ₄ H ₄ O ₄）。溴化物（[C ₄ C ₁im] Br）作为佐剂。为此，首先，在T  = 298.15 K时，通过添加5 wt％IL来确定所研究ABS的二面体曲线和液-液平衡（LLE）数据，以研究其对两相形成的影响。联络线长度（TLL）也已解决。为了评估所研究的ABS对生物分子的提取能力，必需氨基酸L-色氨酸（Trp）被用作分子探针。因此， 在有和没有IL的ABS中，在T = 298.15 K时，确定了Trp的分配系数K _Trp。还研究了盐，PEG分子量和ABS组成对Trp分配系数的影响。发现K _Trp随盐的盐析强度，PEG分子量和ABS组成的增加而增加。此外，在所研究的ABS中，通过向包含PEG 400的ABS中添加5 wt％[C ₄ C ₁ im] Br，可以提高K _Trp值，而对于具有PEG 4000的系统则相反。使用NRTL模型可以将实验LLE和分配系数数据精确关联。