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Irisin Protects Against Hind Limb Ischemia Reperfusion Injury
Drug Design, Development and Therapy ( IF 4.8 ) Pub Date : 2021-02-04 , DOI: 10.2147/dddt.s279318 Ayşegül Küçük 1 , Yücel Polat 2 , Aydan Kılıçarslan 3 , Nuran Süngü 3 , Hakan Kartal 4 , Ali Doğan Dursun 5 , Mustafa Arslan 6
Drug Design, Development and Therapy ( IF 4.8 ) Pub Date : 2021-02-04 , DOI: 10.2147/dddt.s279318 Ayşegül Küçük 1 , Yücel Polat 2 , Aydan Kılıçarslan 3 , Nuran Süngü 3 , Hakan Kartal 4 , Ali Doğan Dursun 5 , Mustafa Arslan 6
Affiliation
Aim: The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R).
Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I–I/R). Irisin (0.5 μg.g− 1, intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal–Wallis and Mann–Whitney U-tests.
Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I–I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase-3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I–I/R groups as compared with those in the control and irisin groups.
Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.
Keywords: irisin, ischemia reperfusion, caspase-3, caspase-8, mice
中文翻译：
鸢尾素可防止后肢缺血再灌注损伤
目的：本研究的目的是评估鸢尾素在小鼠后肢缺血再灌注 (I/R) 模型中的作用。
方法：将小鼠分为四组（每组 n = 6）：对照组、鸢尾素、缺血再灌注（I/R）和鸢尾素缺血再灌注（I-I/R）。在 I/R 手术前 30 分钟给予鸢尾素（0.5 μg.g - 1 ，腹膜内 [ip]）。缺血2小时和再灌注2.5小时后，取血液和组织样本进行生化和组织病理学分析。通过 Kruskal-Wallis 和 Mann-Whitney U检验分析结果。
结果：所有组的总抗氧化状态（TAS）和总氧化状态（TOS）水平存在统计学显着差异。I/R组TAS水平显着低于对照组、鸢尾素组和I-I/R组，而I/R组TOS水平显着高于其他组。与对照组和鸢尾素组相比，I/R 和 I-I/R 组的 Caspase-3 活性和 Caspase-8 活性（炎症指标）显着升高。
结论：鸢尾素可能对骨骼肌缺血再灌注损伤具有保护作用。
关键词：鸢尾素，缺血再灌注，caspase-3，caspase-8，小鼠
更新日期：2021-02-04
Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I–I/R). Irisin (0.5 μg.g− 1, intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal–Wallis and Mann–Whitney U-tests.
Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I–I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase-3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I–I/R groups as compared with those in the control and irisin groups.
Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.
Keywords: irisin, ischemia reperfusion, caspase-3, caspase-8, mice
中文翻译：
鸢尾素可防止后肢缺血再灌注损伤
目的：本研究的目的是评估鸢尾素在小鼠后肢缺血再灌注 (I/R) 模型中的作用。
方法：将小鼠分为四组（每组 n = 6）：对照组、鸢尾素、缺血再灌注（I/R）和鸢尾素缺血再灌注（I-I/R）。在 I/R 手术前 30 分钟给予鸢尾素（0.5 μg.g - 1 ，腹膜内 [ip]）。缺血2小时和再灌注2.5小时后，取血液和组织样本进行生化和组织病理学分析。通过 Kruskal-Wallis 和 Mann-Whitney U检验分析结果。
结果：所有组的总抗氧化状态（TAS）和总氧化状态（TOS）水平存在统计学显着差异。I/R组TAS水平显着低于对照组、鸢尾素组和I-I/R组，而I/R组TOS水平显着高于其他组。与对照组和鸢尾素组相比，I/R 和 I-I/R 组的 Caspase-3 活性和 Caspase-8 活性（炎症指标）显着升高。
结论：鸢尾素可能对骨骼肌缺血再灌注损伤具有保护作用。
关键词：鸢尾素，缺血再灌注，caspase-3，caspase-8，小鼠
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