Non-albicans Candida species are emerging in the nosocomial environment, with the multidrug-resistant (MDR) species Candida auris being the most notorious example. Consequently, rapid and accurate species identification has become essential. The objective of this study was to evaluate five commercially available chromogenic media for the presumptive identification of C. auris. Two novel chromogenic formulations, CHROMagar Candida Plus (CHROMagar) and HiCrome C. auris MDR selective agar (HiMedia), and three reference media, CandiSelect (Bio-Rad), CHROMagar Candida (CHROMagar), and Chromatic Candida (Liofilchem), were inoculated with a collection of 9 genetically diverse C. auris strains and 35 strains from closely related comparator species. After 48 h of incubation, the media were evaluated for their ability to detect and identify C. auris. All media had the same limitations in the differentiation of the more common species Candida dubliniensis and Candida glabrata. Only on CHROMagar Candida Plus did C. auris colonies develop a species-specific coloration. Nevertheless, the closely related pathogenic species Candida pseudohaemulonii and Candida vulturna developed a similar appearance as C. auris on this medium. CHROMagar Candida Plus was shown to be superior in the detection and identification of C. auris, with 100% inclusivity for C. auris compared to 0% and 33% for the reference media and HiCrome C. auris MDR selective agar, respectively. Although C. vulturna and C. pseudohaemulonii can cause false positives, CHROMagar Candida Plus was shown to be a valuable addition to the plethora of mostly molecular methods for C. auris detection and identification.

中文翻译：

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与其他市售制剂相比，两种新型显色培养基用于鉴定耐多​​药耳念珠菌的性能

非白色念珠菌正在医院环境中出现，耐多药 (MDR)念珠菌是最臭名昭著的例子。因此，快速准确的物种识别变得至关重要。本研究的目的是评估五种市售的显色培养基，用于推定鉴定耳念珠菌。两种新型显色制剂 CHROMagar Candida Plus (CHROMagar) 和 HiCrome C. auris MDR 选择性琼脂 (HiMedia)，以及三种参考培养基 CandiSelect (Bio-Rad)、CHROMagar Candida (CHROMagar) 和 Chromatic Candida(Liofilchem)，接种了 9 种遗传多样的C. auris菌株和 35 种来自密切相关的比较物种的菌株。孵育 48 小时后，评估培养基检测和鉴定耳念珠菌的能力。所有培养基在更常见的Candida dubliniensis和Candida glabrata的分化中都具有相同的局限性。只有在 CHROMagar Candida Plus 上，C. auris菌落才会出现特定物种的着色。然而，密切相关的致病菌种假血假丝酵母和外阴假丝酵母的外观与C. auris相似在这个媒体上。科玛嘉念珠菌加上被证明是在检测和识别优越C.耳，用100％为包容性C.耳相比0％，对于参考媒体33％和HiCrome C.耳分别MDR选择性琼脂。尽管C. vulturna和C. pseudohaemulonii会导致假阳性，但 CHROMagar Candida Plus 被证明是对用于C. auris检测和识别的主要分子方法过多的宝贵补充。